IgG antibody

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Donkey anti Rabbit IgG (H/L):Alk. Phos. (Human/Rat/Mouse Adsorbed)

Donkey anti Rabbit IgG (H/L):Biotin (Human/Rat/Mouse Adsorbed)

Donkey anti Rabbit IgG (H/L):FITC (Human/Rat/Mouse Adsorbed)

Donkey anti Rabbit IgG (H/L):HRP (Human/Rat/Mouse Adsorbed)

Donkey anti Rabbit IgG (H/L) (Human/Rat/Mouse Adsorbed)

Donkey anti Rabbit IgG (H/L):RPE (Human/Rat/Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
644004 C E 1 ml
644008 C E 1 mg
644002 F 1 mg
644005 C E 1 ml
644001 C E 1 mg
644009 F 0.5 mg
Donkey anti rabbit IgG (H+L) antibody recognizes rabbit immunoglobulin G heavy and light chains, and shows minimal cross-reactivity with human, rat and mouse serum proteins.
Donkey anti rabbit IgG (H+L) antibody may also recognize the light chains of other rabbit immunoglobulins.

Product Details

Target Species
Rabbit
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Rabbit IgG covalently linked to agarose.
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium azide
50% Glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium azide
Preservative Stabilisers
50% Glycerol
Preservative Stabilisers
None present
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Stabilizing agent
Immunogen
Rabbit IgG.
Approx. Protein Concentrations
0.5 mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date
Shelf Life
18 months from the date of despatch.
Shelf Life
Please see label for expiry date
Shelf Life
18 months from the date of despatch.
Shelf Life
Please see label for expiry date

More Information

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GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/4,000
Immunohistology - Frozen
ELISA 1/5,000 1/20,000
Immunohistology - Frozen
Flow Cytometry Neat 1/10
ELISA 1/4000 1/8000
Immunohistology - Frozen
ELISA
Immunohistology - Frozen
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG antibody

  1. Vega-Flores, G. et al. (2014) The GABAergic septohippocampal pathway is directly involved in internal processes related to operant reward learning.
    Cereb Cortex. 24 (8): 2093-107.
  2. Belostotskaya, G. et al. (2015) Identification of cardiac stem cells within mature cardiac myocytes.
    Cell Cycle. 17 : 1-8.