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IgG antibody

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Goat anti Rabbit IgG(Fc):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
5213-2504
Datasheet
C E WB
MSDS
2 mg loader

Goat anti Rabbit IgG (Fc) antibody recognizes the Fc portion of rabbit IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rabbit IgG, rabbit IgG Fc and rabbit serum. No reaction was observed against rabbit IgG F(ab')2.

Product Details

Target Species
Rabbit
Product Form
Ig Fraction conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution
Use 1.0 ml of sterile DI water. For extended storage reconstitute with 50% glycerol instead of water and then aliquot and store at -20ºC. Avoid freeze thaw cycles.
Preparation
Purified Ig prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Gentamicin sulphate
10 mg/ml Bovine Serum Albumin
Immunogen
Native rabbit IgG.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/110,000
Immunohistology - Frozen 1/1,000 1/5,000
Western Blotting 1/2,000 1/20,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgG antibody

  1. Farr, A. G. & Nakane, P. K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review
    J Immunol Methods 47: 129 - 144
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