IgG antibody
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Goat anti Rabbit IgG (Fc) antibody recognizes the Fc portion of rabbit IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rabbit IgG, rabbit IgG Fc and rabbit serum. No reaction was observed against rabbit IgG F(ab')2.
Product Details
- Target Species
- Rabbit
- Product Form
- Ig Fraction conjugated to Horseradish Peroxidase (HRP) - lyophilised
- Reconstitution
- Use 1.0 ml of sterile DI water. For extended storage reconstitute with 50% glycerol instead of water and then aliquot and store at -20ºC. Avoid freeze thaw cycles.
- Preparation
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Gentamicin sulphate
10 mg/ml Bovine Serum Albumin - Immunogen
- Native rabbit IgG.
Storage Information
- Storage
- This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01870
- Entrez Gene
- LOC100009097
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/110,000 | ||
| Immunohistology - Frozen | 1/1,000 | 1/5,000 | |
| Western Blotting | 1/2,000 | 1/20,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Farr, A. G. & Nakane, P. K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review
J Immunol Methods 47: 129 - 144
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