IgG antibody
Goat anti Rabbit IgG (H/L):HRP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
Horseradish peroxidase conjugated Goat anti Rabbit IgG antibody (5196-2504) used to detect bound polyclonal antibodies in western blotting.
Image caption:
The induction of plasma factors capable of binding gliadin is not detectable in fish fed a 4% wheat gluten diet. Immunoblotting was performed with gliadin protein and mixed plasma extracts from fish fed either the 0 wheat gluten diet (a) or the 4% wheat gluten diet (b). No factors capable of binding to gliadin (30–40 kDa) and inhibiting the binding of anti-gliadin polyclonal antibodies are detectable. Each lane also contains 431 ng recombinant A. carterae protein eIF4E-1A (50 kDa) as a control for loading, transfer efficiency, and specificity of binding. * Statistically different between concentrations.
From: Larkin, M.E.M.; Place, A.R.
Dietary Wheat Gluten Alters the Gut Microbiome and Plasma Taurine Levels in European Sea Bass (Dicentrarchus labrax).
J Mar Sci Eng. 2023, 11, 1022.
doi: 10.3390/jmse11051022.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Rabbit IgG antibody (5198-2504) used to detect bound rabbit antibody by western blotting.
Image caption:
D, Western blotting of P2Y12 protein in platelets of C57BL/6 mice 60 minutes after the injection of thrombin or saline. β-actin was used as a loading control. Optical densitometric analysis was performed using Image J software, and results are expressed in arbitrary units. Results are shown as mean with SD showing individual points; n=4 mice per group. *P=0.0286 Mann-Whitney U test.
From: Bury L, Piselli E, Manni G, Momi S, Ciarrocca Taranta G, Guglielmini G, Fanelli C, De Fano M, Rowley JW, Weyrich AS, Gresele P.
Dicer Neosynthesis Regulates Platelet Reactivity: A Mechanism Altered in Type 2 Diabetes.
Circ Res. 2025;137(6):882-98.
doi: 10.1161/CIRCRESAHA.124.325357.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Rabbit IgG antibody (5198-2504) used to detect bound rabbit antibodies by western blotting.
Image caption:
Thrombin modifies Dicer1 (ribonuclease III enzyme Dicer-1), miR-223-3p (micro RNA 233-3p), P2RY12 (purinergic receptor P2Y12) mRNA, and P2Y12 protein expression in vivo.
H, Western blotting of P2Y12 protein in platelets of Dicer1fl/fl and Dicer1Pf4Δ/Pf4Δ mice 60 minutes after the injection of thrombin or saline. The β-actin was used as a loading control. Optical densitometric analysis was performed using Image J software, and results are expressed in arbitrary units. Results are shown as mean with SD showing individual points; n=5 mice per group for Dicer1fl/fl and n=3 mice per group for Dicer1Pf4Δ/Pf4Δ. #P=2×10−4 saline vs thrombin for Dicer1fl/fl and P=0.5307 saline vs thrombin for Dicer1Pf4Δ/Pf4Δ; the Mann-Whitney U test, with Bonferroni correction for multiple comparisons. Comparison between Dicer1ffl/fl and Dicer1Pf4Δ/Pf4Δ: ++P=0.0055 Dicer1fl/fl vs Dicer1Pf4Δ/Pf4Δ with thrombin and P=0.5337 Dicer1fl/fl vs Dicer1Pf4Δ/Pf4Δ with saline. The Mann-Whitney U test, with Bonferroni correction for multiple comparisons.
From: Bury L, Piselli E, Manni G, Momi S, Ciarrocca Taranta G, Guglielmini G, Fanelli C, De Fano M, Rowley JW, Weyrich AS, Gresele P.
Dicer Neosynthesis Regulates Platelet Reactivity: A Mechanism Altered in Type 2 Diabetes.
Circ Res. 2025;137(6):882-98.
doi: 10.1161/CIRCRESAHA.124.325357.
This image is from an open access article distributed under terms of a Creative Commons Attribution License
Horseradish peroxidase conjugated Goat anti Rabbit IgG antibody (5196-2504) used to detect bound Rabbit anti amyloid precursor protein antibodies by western blotting.
Image caption:
Quantification of APP/Aβ species using immunoblotting. Proteins from RIPA-soluble S1 fractions were separated by SDS-PAGE (20μg per lane) and labelled with the antibody 2B3 against sAPPα (A), Poly8134 against sAPPβ (E), and CT695 against CTFs (I). Densitometric quantification of the bands of interest (arrowheads) was conducted using the Image Lab software and normalisation to total protein loading. For antibody 2B3, three bands at 10 kDa (B), 100 kDa (C) and 17 kDa (D) were identified. For the antibody Poly8134, three bands were quantified at 100 kDa (F), the 50 kDa (G) and 17 kDa (H). The third antibody, CT695 revealed four bands at 75 kDa (J), 50 kDa (K), 25 kDa (L) and 19 kDa (M). Data is shown as individual values, group mean, and SD Data were analysed using 1-way ANOVA with genotype as independent variable and significance is indicated above each graph. No data transformation was required. Males: WT: n = 8 (n = 6 for Poly8134 antibody), mAPPA673T: n = 9, 5×FAD: n = 17 (n = 15 for Poly8134 antibody), 5×FAD × mAPPA673T: n = 14. Abbreviations: crosses, 5×FADx mAPPA673T; ns, not significant; *: loading control.
From: Anschuetz A, Listyono R, Vorley T, Platt B, Harrington CR, Riedel G, Schwab K.
The Icelandic Mutation in the Murine APP Gene, mAPPA673T, on Amyloid-β Plaque Burden in the 5×FAD Alzheimer Model.
J Integr Neurosci. 2026 Jan 22;25(1):48581.
doi: 10.31083/JIN48581.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB Reset| Goat anti Rabbit IgG (H/L) antibody reacts with the heavy and light chains of rabbit IgG, as demonstrated by immunoelectrophoresis. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
- Reconstitution
- Add 1ml of sterile distilled water
- Preparation
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Gentamicin Sulphate 1% Bovine Serum Albumin - Immunogen
- Rabbit IgG, whole molecule.
- Approx. Protein Concentrations
- 2.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature.
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/150,000 | 1/250,000 |
| Immunohistology - Frozen | |
1/500 | 1/2,500 |
| Western Blotting | |
1/10,000 | 1/20,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
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| Description | TMB Signal+ | ||||||
| TMB Core | BUF056A | E | 100 ml | Log in | |||
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| Description | TMB Core | ||||||
| TMB Core+ | BUF062A | E | 100 ml | Log in | |||
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| Description | TMB Core+ | ||||||
References for IgG antibody
-
Martin, M.E.M. & , Place, A.R. (2023) Dietary Wheat Gluten Alters the Gut Microbiome and Plasma Taurine Levels in European Sea Bass (Dicentrarchus labrax)
Journal of Marine Science and Engineering. 11 (5): 1022. -
Bury, L. et al. (2025) Dicer Neosynthesis Regulates Platelet Reactivity: A Mechanism Altered in Type 2 Diabetes.
Circ Res. 137 (6): 882-98. -
Hoffman, E.T. et al. (2025) Aberrant intermediate alveolar epithelial cells promote pathogenic activation of lung fibroblasts in preclinical fibrosis models.
Nat Commun. 16 (1): 8710.
Further Reading
-
Farr, A.G. & Nakane, P.K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review.
J Immunol Methods. 47 (2): 129-44.
- RRID
- AB_619908
- UniProt
- P01870
- P01840
- P01839
- P01841
- P01838
- P01847
- Entrez Gene
- IGKC2
- LOC100009097
- GO Terms
- GO:0003823 antigen binding
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