IgG antibody
Horseradish peroxidase conjugated Goat anti Rabbit IgG antibody (5196-2504) used to detect bound polyclonal antibodies in western blotting.
Image caption:
The induction of plasma factors capable of binding gliadin is not detectable in fish fed a 4% wheat gluten diet. Immunoblotting was performed with gliadin protein and mixed plasma extracts from fish fed either the 0 wheat gluten diet (a) or the 4% wheat gluten diet (b). No factors capable of binding to gliadin (30–40 kDa) and inhibiting the binding of anti-gliadin polyclonal antibodies are detectable. Each lane also contains 431 ng recombinant A. carterae protein eIF4E-1A (50 kDa) as a control for loading, transfer efficiency, and specificity of binding. * Statistically different between concentrations.
From: Larkin, M.E.M.; Place, A.R.
Dietary Wheat Gluten Alters the Gut Microbiome and Plasma Taurine Levels in European Sea Bass (Dicentrarchus labrax).
J Mar Sci Eng. 2023, 11, 1022. doi: 10.3390/jmse11051022.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Rabbit IgG (H/L):HRP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
| Goat anti Rabbit IgG (H/L) antibody reacts with the heavy and light chains of rabbit IgG, as demonstrated by immunoelectrophoresis. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
- Reconstitution
- Add 1ml of sterile distilled water
- Preparation
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Gentamicin Sulphate 1% Bovine Serum Albumin - Immunogen
- Rabbit IgG, whole molecule.
- Approx. Protein Concentrations
- 2.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/150,000 | 1/250,000 |
| Immunohistology - Frozen | |
1/500 | 1/2,500 |
| Western Blotting | |
1/10,000 | 1/20,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TMB Signal+ | ||||||
| TMB Core | BUF056A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TMB Core | ||||||
| TMB Core+ | BUF062A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TMB Core+ | ||||||
References for IgG antibody
-
Martin, M.E.M. & , Place, A.R. (2023) Dietary Wheat Gluten Alters the Gut Microbiome and Plasma Taurine Levels in European Sea Bass (Dicentrarchus labrax)
Journal of Marine Science and Engineering. 11 (5): 1022.
Further Reading
-
Farr, A.G. & Nakane, P.K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review.
J Immunol Methods. 47 (2): 129-44.
- RRID
- AB_619908
- UniProt
- P01870
- P01840
- P01839
- P01841
- P01838
- P01847
- Entrez Gene
- IGKC2
- LOC100009097
- GO Terms
- GO:0003823 antigen binding
View more products with IgG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Rabbit ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up