IgG antibody

Goat anti Rabbit IgG (H/L):FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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405002
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Goat anti Rabbit IgG (H/L) antibody recognises rabbit IgG heavy and light chains and the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry.

Target Species
Rabbit
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to rabbit IgG (H+L) were raised by repeated immunisations of goats with normal rabbit IgG. Purified IgG was prepared from whole serum by affinity chromatography on pooled rabbit IgG covalently linked to agarose. The preparation has been cross absorbed against human and mouse sera and purified paraproteins to minimise potential cross reactivity.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Normal rabbit IgG.
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

References for IgG antibody

  1. Louderback, K.M. et al. (2006) Subbarrel patterns of thalamocortical innervation in rat somatosensory cortical barrels: Organization and postnatal development.
    J Comp Neurol. 497: 32-41.

RRID
AB_609712
UniProt
P01839
P01841
P01838
P01847
P01870
P01840
Entrez Gene
IGKC2
LOC100009097
GO Terms
GO:0003823 antigen binding
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rabbit Products