IgG antibody

100% Secure

Goat anti Rabbit IgG (H/L):FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
405002 F 1 mg
Goat anti Rabbit IgG (H/L) antibody recognises rabbit IgG heavy and light chains and the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry.

Product Details

Target Species
Rabbit
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to rabbit IgG (H+L) were raised by repeated immunisations of goats with normal rabbit IgG. Purified IgG was prepared from whole serum by affinity chromatography on pooled rabbit IgG covalently linked to agarose. The preparation has been cross absorbed against human and mouse sera and purified paraproteins to minimise potential cross reactivity.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Normal rabbit IgG.
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P01839 Related reagents
P01841 Related reagents
P01838 Related reagents
P01847 Related reagents
P01870 Related reagents
P01840 Related reagents
Entrez Gene
IGKC2 Related reagents
LOC100009097 Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Product Specific References

References for IgG antibody

  1. Louderback, K.M. et al. (2006) Subbarrel patterns of thalamocortical innervation in rat somatosensory cortical barrels: Organization and postnatal development.
    J Comp Neurol. 497: 32-41.