Toxoplasma gondii antibody
Rabbit anti Toxoplasma gondii RH Strain antibody used for the identification of Toxoplasma cysts by immunofluorescence.
Image caption:
Compound 2 enhances differentiation. A. Structure of Compound 2. B. Immunofluorescence of a cyst-like structure using an anti-Toxoplasma antibody (green) to detect all parasites and Dolichos biflorus lectin (DL; red) to detect the cyst wall after 72 hr under CO2 starvation conditions. The corresponding differential interference contrast (DIC) and merged images are also shown. Scale bar = 10μm. C. Percentage of DL-positive vacuoles (DL+) in samples treated with either Compound 2 (3μM) or an equivalent volume of DMSO for 72 hr under CO2 starvation conditions (mean ± SD; n = 4 for the wild-type strain and n = 6 for &DELTA;uprt strains). The data were compared using paired Student's t-test (**p < 0.01).
From: Odell AV, Tran F, Foderaro JE, Poupart S, Pathak R, Westwood NJ, et al. (2015) Yeast Three-Hybrid Screen Identifies TgBRADIN/GRA24 as a Negative Regulator of Toxoplasma gondii Bradyzoite Differentiation.
PLoS ONE 10(3): e0120331.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Toxoplasma gondii RH Strain antibody recognizes purified Toxoplasma gondii trophozoites.
T. gondii is an intracellular protozoan parasite that enters its host via the gastrointestinal tract, and is the only member of the Toxoplasma genus. The definitive host of T. gondii is cats, but it can also be carried by a vast number of warm-blooded animals, including humans. Toxoplasmosis is usually minor and self-limiting but can have serious or even fatal effects on a fetus, or if the host is immunocompromised. T. gondii encephalitis (TE) is the most common cerebral opportunistic infection in patients with AIDS.
T. gondii is an intracellular protozoan parasite that enters its host via the gastrointestinal tract, and is the only member of the Toxoplasma genus. The definitive host of T. gondii is cats, but it can also be carried by a vast number of warm-blooded animals, including humans. Toxoplasmosis is usually minor and self-limiting but can have serious or even fatal effects on a fetus, or if the host is immunocompromised. T. gondii encephalitis (TE) is the most common cerebral opportunistic infection in patients with AIDS.
Product Details
- Target Species
- Protozoan
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Toxoplasma gondii RH strain.
- Approx. Protein Concentrations
- IgG concentration 4.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Toxoplasma gondii antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Product Specific References
References for Toxoplasma gondii antibody
-
Parussini, F. et al. (2012) Intramembrane proteolysis of Toxoplasma apical membrane antigen 1 facilitates host-cell invasion but is dispensable for replication.
Proc Natl Acad Sci U S A. 109 (19): 7463-8. -
Odell, A.V. et al. (2015) Yeast Three-Hybrid Screen Identifies TgBRADIN/GRA24 as a Negative Regulator of Toxoplasma gondii Bradyzoite Differentiation.
PLoS One. 10 (3): e0120331. -
Passeri, E. et al. (2016) Infection and characterization of Toxoplasma gondii in human induced neurons from patients with brain disorders and healthy controls.
Microbes Infect. 18 (2): 153-8.
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