Toxoplasma gondii antibody
Rabbit anti Toxoplasma gondii RH Strain antibody used for the identification of Toxoplasma cysts by immunofluorescence.
Image caption:
Compound 2 enhances differentiation. A. Structure of Compound 2. B. Immunofluorescence of a cyst-like structure using an anti-Toxoplasma antibody (green) to detect all parasites and Dolichos biflorus lectin (DL; red) to detect the cyst wall after 72 hr under CO2 starvation conditions. The corresponding differential interference contrast (DIC) and merged images are also shown. Scale bar = 10μm. C. Percentage of DL-positive vacuoles (DL+) in samples treated with either Compound 2 (3μM) or an equivalent volume of DMSO for 72 hr under CO2 starvation conditions (mean ± SD; n = 4 for the wild-type strain and n = 6 for &DELTA;uprt strains). The data were compared using paired Student's t-test (**p < 0.01).
From: Odell AV, Tran F, Foderaro JE, Poupart S, Pathak R, Westwood NJ, et al. (2015) Yeast Three-Hybrid Screen Identifies TgBRADIN/GRA24 as a Negative Regulator of Toxoplasma gondii Bradyzoite Differentiation.
PLoS ONE 10(3): e0120331.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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IF ResetRabbit anti Toxoplasma gondii RH Strain
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Toxoplasma gondii
- Region
- RH STRAIN
Antibody Quality and 
Unrivaled 
| Rabbit anti Toxoplasma gondii RH Strain antibody recognizes purified Toxoplasma gondii trophozoites. T. gondii is an intracellular protozoan parasite that enters its host via the gastrointestinal tract, and is the only member of the Toxoplasma genus. The definitive host of T. gondii is cats, but it can also be carried by a vast number of warm-blooded animals, including humans. Toxoplasmosis is usually minor and self-limiting but can have serious or even fatal effects on a fetus, or if the host is immunocompromised. T. gondii encephalitis (TE) is the most common cerebral opportunistic infection in patients with AIDS. |
- Target Species
- Protozoan
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Toxoplasma gondii RH strain.
- Approx. Protein Concentrations
- IgG concentration 4.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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References for Toxoplasma gondii antibody
-
Parussini, F. et al. (2012) Intramembrane proteolysis of Toxoplasma apical membrane antigen 1 facilitates host-cell invasion but is dispensable for replication.
Proc Natl Acad Sci U S A. 109 (19): 7463-8. -
Odell, A.V. et al. (2015) Yeast Three-Hybrid Screen Identifies TgBRADIN/GRA24 as a Negative Regulator of Toxoplasma gondii Bradyzoite Differentiation.
PLoS One. 10 (3): e0120331. -
Passeri, E. et al. (2016) Infection and characterization of Toxoplasma gondii in human induced neurons from patients with brain disorders and healthy controls.
Microbes Infect. 18 (2): 153-8.
- RRID
- AB_620718
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