IL-1 Alpha antibody

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Rabbit anti Pig Interleukin-1 Alpha:Biotin

Rabbit anti Pig Interleukin-1 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2820B
Datasheet
E
SDS
50 µg loader

AHP2820
Datasheet
E WB
SDS
0.1 mg loader

Rabbit anti Pig Interleukin-1 Alpha recognizes pig interleukin-1 alpha (IL-1 alpha).

IL-1 is a inflammatory cytokine produced by activated macrophages. IL-1 is active in the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. IL-1 acts on Th cells and causes them to secrete IL-2, which induces T cells to undergo mitosis and clonal expansion (Straw Barbara, Diseases of Swine -9th edition).

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to pig interleukin-1 alpha was raised by repeated immunization of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant pig IL-1 ALPHA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P18430
Entrez Gene
IL1A
GO Terms
GO:0001660 fever generation
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005507 copper ion binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0034605 cellular response to heat
GO:0046688 response to copper ion
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of IL-1 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.1 ug/ml 0.5 ug/ml
ELISA 1.0 ug/ml 5.0 ug/ml
Western Blotting 0.1 ug/ml 2.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection reagent in a sandwich ELISA together with AHP2820 as the capture reagent. PPP033 may be used as a standard.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2820B as the detection reagent. PPP033 may be used as a standard.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg