IgG antibody

Goat anti Pig IgG (Fc)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(Fc)

Product Code Applications Pack Size List Price Your Price Qty
AAI41
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E ID WB 1 mg loader
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Goat anti Pig IgG (Fc) antibody recognizes the Fc region of the porcine IgG heavy chains and shows no cross - reactivity with other porcine immunoglobulin classes as evaluated by immunoelectrophoresis. Goat anti Pig IgG (Fc) has not been species cross adsorbed and may react with the Fc region of IgG from other species.

Goat anti Pig IgG (Fc) antibody has been used extensively as a detection reagent for porcine IgG in ELISA, for example monitoring of the IgG response in influenza infected pigs (Crisci et al. 2013).

Target Species
Pig
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to porcine IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified porcine IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Immunodiffusion
Western Blotting 1/1000 1/30,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

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References for IgG antibody

  1. Assana, E. et al. (2010) Antibody responses to the host-protective Taenia solium oncosphere protein TSOL18 in pigs are directed against conformational epitopes.
    Parasite Immunol. 32: 399-405.
  2. Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
    Res Vet Sci. 91 (3): e68-72.
  3. Pyo, H. et al. (2010) Serodiagnosis of porcine reproductive and respiratory syndrome virus infection with the use of glycoprotein 5 antigens.
    Can J Vet Res. 74: 223-7.
  4. Busquets, N. et al. (2010) Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus.
    Vet Res. 41: 74.
  5. Kang, M.L. et al. (2008) Chitosan microspheres containing Bordetella bronchiseptica antigens as novel vaccine against atrophic rhinitis in pigs.
    J Microbiol Biotechnol. 18: 1179-85.
  6. Kim, T.et al. (2009) Bordetella bronchiseptica aroA mutant as a live vaccine vehicle for heterologous porcine circovirus type 2 major capsid protein expression.
    Vet Microbiol. 138: 318-24.
  7. Picherot, M. et al. (2007) Swine infection with Trichinella spiralis: Comparative analysis of the mucosal intestinal and systemic immune responses.
    Vet Parasitol. 143: 122-30.
  8. Tsai, Y.C. et al. (2010) Porcine circovirus type 2 (PCV2) induces cell proliferation, fusion, and chemokine expression in swine monocytic cells in vitro.
    Vet Res. 41: 60.
  9. View The Latest Product References
  10. Scharek, L. et al. (2005) Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets.
    Vet Immunol Immunopathol. 105: 151-61.
  11. Scharek, L. et al. (2007) Impact of the probiotic bacteria Enterococcus faecium NCIMB 10415 (SF68) and Bacillus cereus var. toyoi NCIMB 40112 on the development of serum IgG and faecal IgA of sows and their piglets.
    Arch Anim Nutr. 61: 223-34.
  12. Sheoran, A. et al. (2012) Infection With Cryptosporidium hominis Provides Incomplete Protection of the Host Against Cryptosporidium parvum.
    J Infect Dis 205: 1019-23.
  13. Pina-Pedrero, S. et al. (2012) Genomic and antigenic characterization of monomeric autotransporters of Haemophilus parasuis: an ongoing process of reductive evolution.
    Microbiology. 158: 436-47.
  14. Fleury, A. et al. (2015) Taenia solium: Development of an Experimental Model of Porcine Neurocysticercosis.
    PLoS Negl Trop Dis. 9 (8): e0003980.
  15. Zhang, Y. et al. (2016) Generation of E. coli.-derived virus-like particles of porcine circovirus type 2 and their use in an indirect IgG enzyme-linked immunosorbent assay.
    Arch Virol. 161 (6): 1485-91.
  16. Lee, J.A. et al. (2016) Mycoplasma hyorhinis is a potential pathogen of porcine respiratory disease complex that aggravates pneumonia caused by porcine reproductive and respiratory syndrome virus.
    Vet Immunol Immunopathol. 177: 48-51.
  17. Lorenzen E et al. (2017) Intrauterine inoculation of minipigs with Chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus.
    Microbes Infect. Feb 9. pii: S1286-4579(17)30025-4. [Epub ahead of print]
  18. Zhang, G. et al. (2019) Identification of the B-cell epitopes on N protein of type 2 porcine reproductive and respiratory syndrome virus, using monoclonal antibodies.
    Int J Biol Macromol. 130: 300-6.
  19. Morales, J. et al. (2018) Persistent Taenia solium. Cysticercosis In the State of Morelos, Mexico: Human and Porcine Seroprevalence.
    J Parasitol. 104 (5): 465-72.
  20. Tan, T.K. et al. (2021) A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses.
    Nat Commun. 12 (1): 542.

ELISA

Immunofluorescence

Western Blotting

RRID
AB_323038
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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