IgG antibody

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Goat anti Pig IgG (Fc):Alk. Phos.

Goat anti Pig IgG (Fc):Biotin

Goat anti Pig IgG (Fc):FITC

Goat anti Pig IgG (Fc):HRP

Goat anti Pig IgG (Fc)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI41AB E 0.5 mg
AAI41B E WB 1 mg
AAI41F C F 1 mg
AAI41P E 1 mg
AAI41 E ID WB 1 mg
Goat anti Pig IgG (Fc) antibody recognizes the Fc region of the porcine IgG heavy chains and shows no cross - reactivity with other porcine immunoglobulin classes as evaluated by immunoelectrophoresis. Goat anti Pig IgG (Fc) has not been species cross adsorbed and may react with the Fc region of IgG from other species.

Goat anti Pig IgG (Fc) antibody has been used extensively as a detection reagent for porcine IgG in ELISA, for example monitoring of the IgG response in influenza infected pigs (Crisci et al. 2013).

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG fraction conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to porcine IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.05% Pro-Clean 400
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified porcine IgG.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/50000
ELISA 1:10000 1:100000
Western Blotting 1:10000 1:100000
Flow Cytometry 1/20 1/100
Immunohistology - Frozen 1/20 1/200
ELISA 1/10,000 1/100,000
ELISA 1/100 1/1000
Immunodiffusion
Western Blotting 1/1000 1/30,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Immunofluorescence

Western Blotting

Product Specific References

References for IgG antibody

  1. Assana, E. et al. (2010) Antibody responses to the host-protective Taenia solium oncosphere protein TSOL18 in pigs are directed against conformational epitopes.
    Parasite Immunol. 32: 399-405.
  2. Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
    Res Vet Sci. 91 (3): e68-72.
  3. Pyo, H. et al. (2010) Serodiagnosis of porcine reproductive and respiratory syndrome virus infection with the use of glycoprotein 5 antigens.
    Can J Vet Res. 74: 223-7.
  4. Busquets, N. et al. (2010) Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus.
    Vet Res. 41: 74.
  5. Kang, M.L. et al. (2008) Chitosan microspheres containing Bordetella bronchiseptica antigens as novel vaccine against atrophic rhinitis in pigs.
    J Microbiol Biotechnol. 18: 1179-85.
  6. Kim, T.et al. (2009) Bordetella bronchiseptica aroA mutant as a live vaccine vehicle for heterologous porcine circovirus type 2 major capsid protein expression.
    Vet Microbiol. 138: 318-24.
  7. Picherot, M. et al. (2007) Swine infection with Trichinella spiralis: Comparative analysis of the mucosal intestinal and systemic immune responses.
    Vet Parasitol. 143: 122-30.
  8. Tsai, Y.C. et al. (2010) Porcine circovirus type 2 (PCV2) induces cell proliferation, fusion, and chemokine expression in swine monocytic cells in vitro.
    Vet Res. 41: 60.
  9. Scharek, L. et al. (2005) Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets.
    Vet Immunol Immunopathol. 105: 151-61.
  10. Scharek, L. et al. (2007) Impact of the probiotic bacteria Enterococcus faecium NCIMB 10415 (SF68) and Bacillus cereus var. toyoi NCIMB 40112 on the development of serum IgG and faecal IgA of sows and their piglets.
    Arch Anim Nutr. 61: 223-34.
  11. Sheoran, A. et al. (2012) Infection With Cryptosporidium hominis Provides Incomplete Protection of the Host Against Cryptosporidium parvum.
    J Infect Dis 205: 1019-23.
  12. Pina-Pedrero, S. et al. (2012) Genomic and antigenic characterization of monomeric autotransporters of Haemophilus parasuis: an ongoing process of reductive evolution.
    Microbiology. 158: 436-47.
  13. Fleury, A. et al. (2015) Taenia solium: Development of an Experimental Model of Porcine Neurocysticercosis.
    PLoS Negl Trop Dis. 9 (8): e0003980.
  14. Zhang Y et al. (2016) Generation of E. coli-derived virus-like particles of porcine circovirus type 2 and their use in an indirect IgG enzyme-linked immunosorbent assay.
    Arch Virol. Mar 14. [Epub ahead of print]
  15. Lee, J-A. et al. (2016) Mycoplasma hyorhinis is a potential pathogen of porcine respiratory disease complex that aggravates pneumonia caused by porcine reproductive and respiratory syndrome virus
    Vet Immunol immunopathol. Jun 16 [Epub ahead of print]
  16. Lorenzen E et al. (2017) Intrauterine inoculation of minipigs with Chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus.
    Microbes Infect. Feb 9. pii: S1286-4579(17)30025-4. [Epub ahead of print]