IgA antibody
Horseradish peroxidase conjugated Goat anti porcine IgA antibody (AAI40P) used for the evaluation of C. trachomitis specific serum IgA antibodies in piglets by ELISA.
Image caption:
Serum antibodies. Detection of serum antibodies against C. trachomatis, measured as the difference in OD values day (x) and day 0.
From: Erneholm K, Lorenzen E, Bøje S, Olsen AW, Andersen P, Cassidy JP, Follmann F, Jensen HE, Agerholm JS.
Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D.
BMC Vet Res. 2016 Sep 10;12(1):200.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Horseradish peroxidase conjugated Goat anti Porcine IgA antibody (AAI40P) used for the evaluation of Va HRV specific antibodies in piglet sera by ELISA.
Image caption:
Wa HRV specific porcine Abs in piglet's sera during the experience. The isotype-specific and VN Abs to Wa HRV in serum samples were determined weekly by ELISA and VN assay. The arrow indicates the experimental inoculation with VirWa HRV (0 PID). PID: post inoculation days.
From: Vega CG, Bok M, Vlasova AN, Chattha KS, Fernández FM, Wigdorovitz A, et al. (2012)
IgY Antibodies Protect against Human Rotavirus Induced Diarrhea in the Neonatal Gnotobiotic Piglet Disease Model.
PLoS ONE 7(8): e42788.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Porcine IgA antibody (AAI40P) used for the evaluation of IgA levels in serum and nasal swabs following influenza infection by ELISA.
Image caption:
Antibodies in serum and nasal swabs. (A) Influenza total anti-NP antibodies in serum were analyzed by ELISA competition assay. (B) Influenza IgA anti-NP antibodies in nasal swabs were analyzed by ELISA at day 0, 21 and 28 PI. White squares, white rhomboids, white triangles and black circles represent average values from animals belonging to Mock/Mock (□), SwH1N1/Mock (◇), Mock/pH1N1 (△) and SwH1N1/pH1N1 (●) group, respectively. Error bars represent one SD above and below the mean.
From: Busquets N, Segalés J, Córdoba L, Mussá T, Crisci E, Martín-Valls GE, Simon-Grifé M, Pérez-Simó M, Pérez-Maíllo M, Núñez JI, Abad FX, Fraile L, Pina S, Majó N, Bensaid A, Domingo M, Montoya M.
Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus.
Vet Res. 2010 Sep-Oct;41(5):74.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Porcine IgA antibody (AAI40P) used for the evaluation of IgA levels in serum and nasal swabs following influenza infection by ELISA.
Image caption:
IgA and IgG antibodies in serum. (A) Influenza IgA and (B) IgG anti-NP antibodies in serum were analyzed by ELISA at day 0, 21 and 28 PI. White squares, white rhomboids, white triangles and black circles represent mean values from animals belonging to Mock/Mock (□), SwH1N1/Mock (◇), Mock/pH1N1 (△) and SwH1N1/pH1N1 (●) groups, respectively. Error bars represent one SD above and below the mean.
From: Busquets N, Segalés J, Córdoba L, Mussá T, Crisci E, Martín-Valls GE, Simon-Grifé M, Pérez-Simó M, Pérez-Maíllo M, Núñez JI, Abad FX, Fraile L, Pina S, Majó N, Bensaid A, Domingo M, Montoya M.
Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus.
Vet Res. 2010 Sep-Oct;41(5):74.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
HRP conjugated Goat anti Pig IgA antibody (AAI40P) used as a detection reagent in a sandwich ELISA to evaluate IgA levels in porcine body fluids.
Image caption:
Pigs (n = 3 in each group) were dosed twice (prime and boost) IM, 4 weeks apart with 5 or 50μg RBD-SpyVLP or 100μg of spike glycoprotein, all adjuvanted with AddaVax and sera were harvested at indicated time-points and analysed in a ELISA against RBD, Antibody levels in e nasal and f oral swabs were measured at indicated time points using RBD ELISA. Data are presented as the group mean ± 1 SEM. Black arrows indicate when the vaccines were administered. Kruskal–Wallis test was used to compare data on day 56 in e, n.s. not significant. *p <0.05 and data on day 56 were compared to convalescent human plasma using two-tailed Mann–Whitney U Test.
From: Tan, T.K., Rijal, P., Rahikainen, R. et al..
A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses.
Nat Commun 12, 542 (2021).
doi: 10.1038/s41467-020-20654-7
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Pig IgA
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgA
Antibody Quality and Performance Guarantee
| Goat anti pig IgA antibody recognizes porcine IgA and shows no cross-reactivity with other porcine immunoglobulin classes as assessed by immunoelectrophoresis. This antibody may cross-react with IgA from other species. Goat anti Porcine IgA antibody has been succesfully used for the evaluation of porcine IgA levels in body fluids of pigs by both ELISA and Western blotting. |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to porcine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified porcine IgA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/100 | 1/30000 |
| Immunodiffusion | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
References for IgA antibody
-
Takahashi, M. et al (2005) Correlation between positivity for immunoglobulin A antibodies and viraemia of swine hepatitis E virus observed among farm pigs in Japan.
J Gen Virol. 86: 1807-13. -
Scharek, L. et al. (2005) Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets.
Vet Immunol Immunopathol. 105: 151-61. -
Nakai, I. et al. (2006) Different fecal shedding patterns of two common strains of hepatitis E virus at three Japanese swine farms.
Am J Trop Med Hyg. 75: 1171-7. -
Zhang, L. et al. (2007) Intranasal administration of CpG oligonucleotides induces mucosal and systemic Type 1 immune responses and adjuvant activity to porcine reproductive and respiratory syndrome killed virus vaccine in piglets in vivo.
Int Immunopharmacol. 7: 1732-40. -
Bestagno, M. et al. (2007) Recombinant dimeric small immunoproteins neutralize transmissible gastroenteritis virus infectivity efficiently in vitro and confer passive immunity in vivo.
J Gen Virol. 88: 187-95. -
Bestagno, M. et al. (2007) Recombinant dimeric small immunoproteins neutralize transmissible gastroenteritis virus infectivity efficiently in vitro and confer passive immunity in vivo.
J Gen Virol. 88: 187-95. -
Picherot, M. et al. (2007) Swine infection with Trichinella spiralis: Comparative analysis of the mucosal intestinal and systemic immune responses.
Vet Parasitol. 143: 122-30. -
Scharek, L. et al. (2007) Impact of the probiotic bacteria Enterococcus faecium NCIMB 10415 (SF68) and Bacillus cereus var. toyoi NCIMB 40112 on the development of serum IgG and faecal IgA of sows and their piglets.
Arch Anim Nutr. 61: 223-34.
View The Latest Product References
-
Eblé, P.L. et al. (2007) Serological and mucosal immune responses after vaccination and infection with FMDV in pigs.
Vaccine. 25: 1043-54. -
Kang, M.L. et al. (2008) Chitosan microspheres containing Bordetella bronchiseptica antigens as novel vaccine against atrophic rhinitis in pigs.
J Microbiol Biotechnol. 18: 1179-85. -
Linghua, Z. et al. (2008) In vivo oral administration effects of various oligodeoxynucleotides containing synthetic immunostimulatory motifs in the immune response to pseudorabies attenuated virus vaccine in newborn piglets.
Vaccine. 26 (2): 224-33. -
Olvera, A. et al. (2010) Virulence-associated trimeric autotransporters of Haemophilus parasuis are antigenic proteins expressed in vivo.
Vet Res. 41: 26. -
Sheoran A et al. (2012) Infection with Cryptosporidium hominis provides incomplete protection of the host against Cryptosporidium parvum.
J Infect Dis. 205 (6): 1019-23. -
Cordes, H. et al. (2012) Cell-mediated and humoral immune responses in pigs following primary and challenge-exposure to Lawsonia intracellularis.
Vet Res. 43:9. -
Crisci, E. et al. (2014) Immune characterization of long pentraxin 3 in pigs infected with influenza virus.
Vet Microbiol. 168 (1): 185-92. -
Le Bourgot, C. et al. (2016) Short-chain fructooligosaccharide supplementation during gestation and lactation or after weaning differentially impacts pig growth and IgA response to influenza vaccination
Journal of Functional Foods. 24: 307-15. -
Lorenzen, E. et al. (2017) Intrauterine inoculation of minipigs with Chlamydia trachomatis. during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus.
Microbes Infect. 19 (6): 334-42. -
Williams, A.R. et al. (2017) Dietary cinnamaldehyde enhances acquisition of specific antibodies following helminth infection in pigs.
Vet Immunol Immunopathol. 189: 43-52. -
Williams, A.R. et al. (2017) A polyphenol-enriched diet and Ascaris suum. infection modulate mucosal immune responses and gut microbiota composition in pigs.
PLoS One. 12 (10): e0186546. -
López-Serrano, S. et al. (2021) Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis. Modulates Immunity Traits in Their Offspring.
Vaccines. 9 (5): 534. -
Tan, T.K. et al. (2021) A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses.
Nat Commun. 12 (1): 542. -
López-Serrano, S. et al. (2023) Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulent Glaesserella parasuis adjuvanted with CAF®01 or CDA.
Vaccine X. 14: 100330. -
Michael, H. et al. (2020) Malnutrition Decreases Antibody Secreting Cell Numbers Induced by an Oral Attenuated Human Rotavirus Vaccine in a Human Infant Fecal Microbiota Transplanted Gnotobiotic Pig Model.
Front Immunol. 11: 196.
- RRID
- AB_323041
AAI40
149207
153411
164367
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