IgA antibody

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Goat anti Pig IgA:Alk. Phos.

Goat anti Pig IgA:Biotin

Goat anti Pig IgA:FITC

Goat anti Pig IgA:HRP

Goat anti Pig IgA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAI40AB
Datasheet
C E WB
MSDS
0.5 mg loader

AAI40B
Datasheet
E WB
MSDS
1 mg loader

AAI40F
Datasheet
C F
MSDS
1 mg loader

AAI40P
Datasheet
E
MSDS
1 mg loader

AAI40
Datasheet
E ID
MSDS
1 mg loader

Goat anti pig IgA antibody recognizes porcine IgA and shows no cross-reactivity with other porcine immunoglobulin classes as assessed by immunoelectrophoresis. This antibody may cross-react with IgA from other species.

Goat anti Porcine IgA antibody has been succesfully used for the evaluation of porcine IgA levels in body fluids of pigs by both ELISA and Western blotting.

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to porcine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES
0.1M NaCl
1mM MgCl2
0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.05% Proclin™ 300
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified porcine IgA.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Immunohistology - Frozen 1/100 1/1000
Western Blotting 1/1000 1/10000
ELISA 1:10000 1:100000
Western Blotting 1:10000 1:100000
Flow Cytometry
Immunohistology - Frozen 1/50 1/500
ELISA 1/10,000 1/100,000
ELISA 1/100 1/30000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

Western Blotting

Product Specific References

References for IgA antibody

  1. Takahashi, M. et al (2005) Correlation between positivity for immunoglobulin A antibodies and viraemia of swine hepatitis E virus observed among farm pigs in Japan.
    J Gen Virol. 86: 1807-13.
  2. Linghua, Z. et al. (2008) In vivo oral administration effects of various oligodeoxynucleotides containing synthetic immunostimulatory motifs in the immune response to pseudorabies attenuated virus vaccine in newborn piglets.
    Vaccine. 26 (2): 224-33.
  3. Olvera, A. et al. (2010) Virulence-associated trimeric autotransporters of Haemophilus parasuis are antigenic proteins expressed in vivo.
    Vet Res. 41: 26.
  4. Scharek, L. et al. (2005) Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets.
    Vet Immunol Immunopathol. 105: 151-61.
  5. Scharek, L. et al. (2007) Impact of the probiotic bacteria Enterococcus faecium NCIMB 10415 (SF68) and Bacillus cereus var. toyoi NCIMB 40112 on the development of serum IgG and faecal IgA of sows and their piglets.
    Arch Anim Nutr. 61: 223-34.
  6. Eblé, P.L. et al. (2007) Serological and mucosal immune responses after vaccination and infection with FMDV in pigs.
    Vaccine. 25: 1043-54.
  7. Bestagno, M. et al. (2007) Recombinant dimeric small immunoproteins neutralize transmissible gastroenteritis virus infectivity efficiently in vitro and confer passive immunity in vivo.
    J Gen Virol. 88: 187-95.
  8. Nakai, I. et al. (2006) Different fecal shedding patterns of two common strains of hepatitis E virus at three Japanese swine farms.
    Am J Trop Med Hyg. 75: 1171-7.
  9. Kang, M.L. et al. (2008) Chitosan microspheres containing Bordetella bronchiseptica antigens as novel vaccine against atrophic rhinitis in pigs.
    J Microbiol Biotechnol. 18: 1179-85.
  10. Picherot, M. et al. (2007) Swine infection with Trichinella spiralis: Comparative analysis of the mucosal intestinal and systemic immune responses.
    Vet Parasitol. 143: 122-30.
  11. Sheoran A et al. (2012) Infection with Cryptosporidium hominis provides incomplete protection of the host against Cryptosporidium parvum.
    J Infect Dis. 205 (6): 1019-23.
  12. Bestagno, M. et al. (2007) Recombinant dimeric small immunoproteins neutralize transmissible gastroenteritis virus infectivity efficiently in vitro and confer passive immunity in vivo.
    J Gen Virol. 88: 187-95.
  13. Cordes, H. et al. (2012) Cell-mediated and humoral immune responses in pigs following primary and challenge-exposure to Lawsonia intracellularis.
    Vet Res. 43:9.
  14. Crisci, E. et al. (2014) Immune characterization of long pentraxin 3 in pigs infected with influenza virus.
    Vet Microbiol. 168 (1): 185-92.
  15. Le Bourgot, C, et al. (2016) Short-chain fructooligosaccharide supplementation during gestation and lactation or after weaning differentially impacts pig growth and IgA response to influenza vaccination
    J Funct Foods. 24: 307-15.
  16. Lorenzen, E. et al. (2017) Intrauterine inoculation of minipigs with Chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus.
    Microbes Infect. Feb 9. pii: S1286-4579(17)30025-4. [Epub ahead of print]
  17. Williams, A.R. et al. (2017) Dietary cinnamaldehyde enhances acquisition of specific antibodies following helminth infection in pigs.
    Vet Immunol Immunopathol. 189: 43-52.
  18. Williams, A.R. et al. (2017) A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs.
    PLoS One. 12 (10): e0186546.