Dynorphin B antibody

Rabbit anti Dynorphin B (aa1-13)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Dynorphin B
Region
(aa1-13)

Product Code Applications Pack Size List Price Your Price Qty
AHP377
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C R 0.1 ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Dynorphin B antibody recognizes dynorphin B, an endogenous opioid peptide, also known as rimorphin with the peptide sequence Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr, (YGGFLRRQFKVVT) derived from the sequential cleavage of pro-enkephalin B to form leumorphin which is, in turn cleaved to form dynorphin B (Devi et al. 1985). The cleavage of the dynorphin precursors to form the dynorphin neuropeptides is largely controlled by the serine protease cathepsin L which co-localizes with dynorphins in secretory vesicles of brain cortical neurons (Funkelstein et al. 2010) and prohormone convertase 2, a member of the furin, peptidate S8 family (Schwarzer 2009).

Dynorphin b sequence is well conserved among mammal, avian (Youngren et al. 1993) and reptilian (Goldsmith et al. 1992) species. Rabbit anti Dynorphin b antibody is expected to have a wide species cross reactivity.

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Chimpanzee Expected from Sequence
Dog Expected from Sequence
Gorilla Expected from Sequence
Horse Expected from Sequence
Human Expected from Sequence
Mouse Expected from Sequence
Rhesus Monkey
Hamster
Rat
Guinea Pig
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to Dynorphin B (1-13) were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide, YGGFLRRQFKVVT, corresponding to full-length porcine dynorphin B (1-13), conjugated to thyroglobulin.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/250 1/2500
Radioimmunoassays 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Dynorphin B antibody

  1. Khachaturian, H. et al. (1985) Prodynorphin peptide immunocytochemistry in rhesus monkey brain.
    Peptides. 6 Suppl 2: 155-66.
  2. Sherman, T.G. et al. (1988) Regulation of hypothalamic magnocellular neuropeptides and their mRNAs in the Brattleboro rat: coordinate responses to further osmotic challenge.
    J Neurosci. 8 (10): 3785-96.
  3. NealCr, J.r. & Newman, S.W. (1989) Prodynorphin peptide distribution in the forebrain of the Syrian hamster and rat: a comparative study with antisera against dynorphin A, dynorphin B, and the C-terminus of the prodynorphin precursor molecule.
    J Comp Neurol. 288 (3): 353-86.
  4. Zhang, L. et al. (2004) Cocaine-induced intracellular signaling and gene expression are oppositely regulated by the dopamine D1 and D3 receptors.
    J Neurosci. 24: 3344-54.
  5. Darmopil, S. et al. (2009) Genetic inactivation of dopamine D1 but not D2 receptors inhibits L-DOPA-induced dyskinesia and histone activation.
    Biol Psychiatry. 66: 603-13.
  6. Ruiz-DeDiego, I. et al. (2014) Activation of DREAM (Downstream Regulatory Element Antagonistic Modulator), a Calcium-Binding Protein, Reduces L-DOPA-Induced Dyskinesias in Mice.
    Biol Psychiatry. pii: S0006-3223(14)00224-8.
  7. Solís, O. et al. (2021) Behavioral sensitization and cellular responses to psychostimulants are reduced in D2R knockout mice.
    Addict Biol. 26 (1): e12840.
  8. Agostinho, A.S. et al. (2019) Dynorphin-based "release on demand" gene therapy for drug-resistant temporal lobe epilepsy.
    EMBO Mol Med. 11 (10): e9963.
  9. View The Latest Product References
  10. Hernández, L.F. et al. (2017) Striatal activation by optogenetics induces dyskinesias in the 6-hydroxydopamine rat model of Parkinson disease.
    Mov Disord. 32 (4): 530-7.
  11. Darmopil, S. et al. (2008) Tyrosine hydroxylase cells appearing in the mouse striatum after dopamine denervation are likely to be projection neurones regulated by L-DOPA.
    Eur J Neurosci. 27 (3): 580-92.

RRID
AB_322029
UniProt
P01214
Entrez Gene
PDYN
GO Terms
GO:0001515 opioid peptide activity
GO:0005576 extracellular region
GO:0007218 neuropeptide signaling pathway
GO:0007268 synaptic transmission
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