Dynorphin B antibody
Rabbit anti Porcine dynorphin B antibody (AHP377) used to demonstrate dynorphin staining in murine brain by immunohistochemistry on formalin fixed vibrotome sections.
Image caption:
Distribution of vector‐derived dynorphins
A–D: Immunohistochemistry was performed on PFA‐fixed 40‐μm sections obtained from a pDyn knockout mouse unilaterally injected with 2 × 109 gp of AAV‐pDyn 3 weeks before. The ipsilateral hippocampus (A) displays strong immunoreactivity in the terminal field of mossy fibers and the polymorph cell layer, indicative for expression in granule cells and localization in granule cell axons. Strong immunoreactivity was also observed in the inner molecular layer in both hippocampi (A, B). This region is strongly innervated by mossy cells, located in the hilus and forming strong projections also contralaterally. Several non‐principal neurons were labeled in the ipsilateral polymorph cell layer (C). These cells may represent different types of GABAergic interneurons beside the glutamatergic mossy cells. Labeling in the molecular layer is dense in the supergranular band but faint in the middle and outer layers. Some well‐labeled filaments (presumably fibers due to their varicose structure) are visible (D). The diffuse light labeling in the ipsilateral but not contralateral molecular layer might be due to Dyn stored in dendrites, as suggested by some studies, or represent axon terminals of GABAergic interneurons. Scale bars represent 500 μm (A) and (B); 100 μm (C); and 50 μm (D). Box in (A) delineates the section enlarged in (C). Box in (C) delineates the section enlarged in (D). Immunohistochemistry was performed using rabbit anti‐human dynorphin B antibodies (Serotec AHP377; 1: 1,000) and goat anti‐rabbit secondary antibodies coupled to horseradish peroxidase (Dako P 0448; 1: 400) followed by 3,3′‐diaminobenzidine staining.
From: Agostinho AS, Mietzsch M, Zangrandi L, Kmiec I, Mutti A, Kraus L, Fidzinski P, Schneider UC, Holtkamp M, Heilbronn R, Schwarzer C.
Dynorphin-based "release on demand" gene therapy for drug-resistant temporal lobe epilepsy.
EMBO Mol Med. 2019 Oct;11(10):e9963.
doi: 10.15252/emmm.201809963.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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C ResetRabbit anti Dynorphin B (aa1-13)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Dynorphin B
- Region
- (aa1-13)
Antibody Quality and 
Unrivaled 
| Rabbit anti Dynorphin B antibody recognizes dynorphin B, an endogenous opioid peptide, also known as rimorphin with the peptide sequence Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr, (YGGFLRRQFKVVT) derived from the sequential cleavage of pro-enkephalin B to form leumorphin which is, in turn cleaved to form dynorphin B (Devi et al. 1985). The cleavage of the dynorphin precursors to form the dynorphin neuropeptides is largely controlled by the serine protease cathepsin L which co-localizes with dynorphins in secretory vesicles of brain cortical neurons (Funkelstein et al. 2010) and prohormone convertase 2, a member of the furin, peptidate S8 family (Schwarzer 2009).
Dynorphin b sequence is well conserved among mammal, avian (Youngren et al. 1993) and reptilian (Goldsmith et al. 1992) species. Rabbit anti Dynorphin b antibody is expected to have a wide species cross reactivity. |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Chimpanzee Expected from Sequence Dog Expected from Sequence Gorilla Expected from Sequence Horse Expected from Sequence Human Expected from Sequence Mouse Expected from Sequence Rhesus Monkey Hamster Rat Guinea Pig Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to Dynorphin B (1-13) were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide, YGGFLRRQFKVVT, corresponding to full-length porcine dynorphin B (1-13), conjugated to thyroglobulin.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
1/250 | 1/2500 |
| Radioimmunoassays | |
1/5000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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|---|---|---|---|---|---|---|---|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
References for Dynorphin B antibody
-
Khachaturian, H. et al. (1985) Prodynorphin peptide immunocytochemistry in rhesus monkey brain.
Peptides. 6 Suppl 2: 155-66. -
Sherman, T.G. et al. (1988) Regulation of hypothalamic magnocellular neuropeptides and their mRNAs in the Brattleboro rat: coordinate responses to further osmotic challenge.
J Neurosci. 8 (10): 3785-96. -
NealCr, J.r. & Newman, S.W. (1989) Prodynorphin peptide distribution in the forebrain of the Syrian hamster and rat: a comparative study with antisera against dynorphin A, dynorphin B, and the C-terminus of the prodynorphin precursor molecule.
J Comp Neurol. 288 (3): 353-86. -
Zhang, L. et al. (2004) Cocaine-induced intracellular signaling and gene expression are oppositely regulated by the dopamine D1 and D3 receptors.
J Neurosci. 24: 3344-54. -
Darmopil, S. et al. (2009) Genetic inactivation of dopamine D1 but not D2 receptors inhibits L-DOPA-induced dyskinesia and histone activation.
Biol Psychiatry. 66: 603-13. -
Ruiz-DeDiego, I. et al. (2014) Activation of DREAM (Downstream Regulatory Element Antagonistic Modulator), a Calcium-Binding Protein, Reduces L-DOPA-Induced Dyskinesias in Mice.
Biol Psychiatry. pii: S0006-3223(14)00224-8. -
Solís, O. et al. (2021) Behavioral sensitization and cellular responses to psychostimulants are reduced in D2R knockout mice.
Addict Biol. 26 (1): e12840. -
Agostinho, A.S. et al. (2019) Dynorphin-based "release on demand" gene therapy for drug-resistant temporal lobe epilepsy.
EMBO Mol Med. 11 (10): e9963.
View The Latest Product References
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Hernández, L.F. et al. (2017) Striatal activation by optogenetics induces dyskinesias in the 6-hydroxydopamine rat model of Parkinson disease.
Mov Disord. 32 (4): 530-7. -
Darmopil, S. et al. (2008) Tyrosine hydroxylase cells appearing in the mouse striatum after dopamine denervation are likely to be projection neurones regulated by L-DOPA.
Eur J Neurosci. 27 (3): 580-92.
- RRID
- AB_322029
- UniProt
- P01214
- Entrez Gene
- PDYN
- GO Terms
- GO:0001515 opioid peptide activity
- GO:0005576 extracellular region
- GO:0007218 neuropeptide signaling pathway
- GO:0007268 synaptic transmission
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