Dynorphin B antibody
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Rabbit anti Dynorphin B antibody recognizes dynorphin B, an endogenous opioid peptide, also known as rimorphin with the peptide sequence Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr, derived from the sequential cleavage of pro-enkephalin B to form leumorphin which is, in turn cleaved to form dynorphin B (Devi et al. 1985). The cleavage of the dynorphin precursors to form the dynorphin neuropeptides is largely controlled by the serine protease cathepsin L which co-localizes with dynorphins in secretory vesicles of brain cortical neurons(Funkelstein et al. 2010) and prohormone convertase 2, a member of the furin, peptidate S8 family (Schwarzer 2009).
Dynorphin b sequence is well conserved among mammal, avian (Youngren et al. 1993) and reptilian (Goldsmith et al. 1992) species. Rabbit anti Dynorphin b antibody is expected to have a wide species cross reactivity.
Dynorphin b sequence is well conserved among mammal, avian (Youngren et al. 1993) and reptilian (Goldsmith et al. 1992) species. Rabbit anti Dynorphin b antibody is expected to have a wide species cross reactivity.
Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Chimpanzee Expected from Sequence Dog Expected from Sequence Gorilla Expected from Sequence Horse Expected from Sequence Human Expected from Sequence Mouse Expected from Sequence Rhesus Monkey Hamster Rat Guinea Pig Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to Dynorphin B (1-13) were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide, YGGFLRRQFKVVT, corresponding to full-length porcine dynorphin B (1-13), conjugated to thyroglobulin.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01214
- Entrez Gene
- PDYN
- GO Terms
- GO:0001515 opioid peptide activity
- GO:0005576 extracellular region
- GO:0007218 neuropeptide signaling pathway
- GO:0007268 synaptic transmission
- Regulatory
- For research purposes only
Applications of Dynorphin B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/250 | 1/2500 | |
| Radioimmunoassays | 1/5000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for Dynorphin B antibody
-
Khachaturian, H. et al. (1985) Prodynorphin peptide immunocytochemistry in rhesus monkey brain.
Peptides. 6 Suppl 2: 155-66. -
Sherman, T.G. et al. (1988) Regulation of hypothalamic magnocellular neuropeptides and their mRNAs in the Brattleboro rat: coordinate responses to further osmotic challenge.
J Neurosci. 8 (10): 3785-96. -
NealCr, J.r. & Newman, S.W. (1989) Prodynorphin peptide distribution in the forebrain of the Syrian hamster and rat: a comparative study with antisera against dynorphin A, dynorphin B, and the C-terminus of the prodynorphin precursor molecule.
J Comp Neurol. 288 (3): 353-86. -
Zhang, L. et al. (2004) Cocaine-induced intracellular signaling and gene expression are oppositely regulated by the dopamine D1 and D3 receptors.
J Neurosci. 24: 3344-54. -
Darmopil, S. et al. (2009) Genetic inactivation of dopamine D1 but not D2 receptors inhibits L-DOPA-induced dyskinesia and histone activation.
Biol Psychiatry. 66: 603-13. -
Ruiz-DeDiego, I. et al. (2014) Activation of DREAM (Downstream Regulatory Element Antagonistic Modulator), a Calcium-Binding Protein, Reduces L-DOPA-Induced Dyskinesias in Mice.
Biol Psychiatry. pii: S0006-3223(14)00224-8. -
Solís, O. et al. (2021) Behavioral sensitization and cellular responses to psychostimulants are reduced in D2R knockout mice.
Addict Biol. 26 (1): e12840.
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