Rabbit anti Phosphoserine antibody recognises phosphoserine, peptidyl-phosphoserine, and serine-phosphorylated proteins. Rabbit anti Phosphoserine antibody does not cross react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross reactivity with free phosphothreonine is observed.
Phosphorylation and dephosphorylation of intracellular proteins occurs in response to extracellular signals. In certain disease states such as cancer, abnormal protein phosphorylation can occur.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to phosphoserine was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate Buffered Saline
- Preservative Stabilisers
- 50% Glycerol
- Keyhole Limpet Haemocyanin - phosphoserine conjugates
- Approx. Protein Concentrations
- IgG concentration 0.25 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Phosphoserine antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available