M-CSF antibody

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Goat anti Mouse M-CSF

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AAM75 Datasheet	E FN WB SDS	0.1 mg	Log in		Get Quote

Goat anti Mouse M-CSF antibody recognizes mouse M-CSF (Macrophage colony-stimulating factor), a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.

Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor). A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodelling.

Product Details

Target Species: Mouse
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Antiserum Preparation: Antiserum to mouse M-CSF was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Carrier Free: Yes
Immunogen: Recombinant mouse M-CSF.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P07141
Entrez Gene: Csf1
GO Terms: GO:0005886 plasma membrane; GO:0001954 positive regulation of cell-matrix adhesion; GO:0003006 developmental process involved in reproduction; GO:0016021 integral to membrane; GO:0005125 cytokine activity; GO:0005157 macrophage colony-stimulating factor receptor binding; GO:0005615 extracellular space; GO:0008083 growth factor activity; GO:0030278 regulation of ossification; GO:0030335 positive regulation of cell migration; GO:0040018 positive regulation of multicellular organism growth; GO:0042488 positive regulation of odontogenesis of dentine-containing tooth; GO:0042803 protein homodimerization activity; GO:0043235 receptor complex; GO:0045651 positive regulation of macrophage differentiation; GO:0045657 positive regulation of monocyte differentiation; GO:0045672 positive regulation of osteoclast differentiation; GO:0045860 positive regulation of protein kinase activity; GO:0046579 positive regulation of Ras protein signal transduction; GO:0048873 homeostasis of number of cells within a tissue; GO:0060444 branching involved in mammary gland duct morphogenesis; GO:0060611 mammary gland fat development; GO:0060763 mammary duct terminal end bud growth
Regulatory: For research purposes only

Applications of M-CSF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.5	2.0ug/ml
Functional Assays	0.05	0.08ug/ml
Western Blotting	0.1	0.2ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

Product Specific References

References for M-CSF antibody

Djelloul, M. et al. (2016) RAE-1 expression is induced during experimental autoimmune encephalomyelitis and is correlated with microglia cell proliferation.
Brain Behav Immun. 58: 209-17.

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