Goat anti Mouse M-CSF antibody recognizes mouse M-CSF (Macrophage colony-stimulating factor), a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.
Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor). A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodelling.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
- Antiserum Preparation
- Antiserum to mouse M-CSF was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant mouse M-CSF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
positive regulation of cell-matrix adhesion
developmental process involved in reproduction
integral to membrane
macrophage colony-stimulating factor receptor binding
growth factor activity
regulation of ossification
positive regulation of cell migration
positive regulation of multicellular organism growth
positive regulation of odontogenesis of dentine-containing tooth
protein homodimerization activity
positive regulation of macrophage differentiation
positive regulation of monocyte differentiation
positive regulation of osteoclast differentiation
positive regulation of protein kinase activity
positive regulation of Ras protein signal transduction
homeostasis of number of cells within a tissue
branching involved in mammary gland duct morphogenesis
mammary gland fat development
mammary duct terminal end bud growth
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of M-CSF antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for M-CSF antibody
Djelloul, M. et al. (2016) RAE-1 expression is induced during experimental autoimmune encephalomyelitis and is correlated with microglia cell proliferation.
Brain Behav Immun. 58: 209-17.