CXCL16 antibody

100% Secure

Goat anti Mouse CXCL16:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2804Z
Datasheet
E WB
SDS
0.1 mg loader

Goat anti Mouse CXCL16 antibody, recognizes C-X-C motif Chemokine 16 (CXCL16). Unlike most chemokines, CXCL16 is a membrane bound ligand anchored through transmembrane domains. Although a soluble form of CXCL16 is generated through proteolytic cleavage. Transmembrane CXCL16 is mainly expressed on macrophages, dendritic cells, B cells and various tumor cells. In this form, it acts as an adhesion molecule though the binding of its receptor CXCR6 expressed on other cells including T cells. Transmembrane CXCL16 also binds gram positive and gram negative bacteria, playing a role in innate and adaptive immune responses (Shimaoka et al. 2003).

Soluble CXCL16 has chemoattractant activity, and elicits this response through the receptor CXCR6.There is also mounting evidence suggesting an additional role in tumor progression. With a number of studies suggesting CXCL16 as a biomarker for the evaluation of some tumor type prognoses (Richardsen et al. 2015, Shibata et al. 2020, Gooden et al. 2014).

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Antiserum Preparation
Produced from sera of goats immunized with recombinant mouse CXCL16 and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
E. coli derived recombinant mouse CXCL16
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/μg of protein (< 1 EU/μg)

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q8BSU2
Entrez Gene
Cxcl16
GO Terms
GO:0016021 integral to membrane
GO:0005041 low-density lipoprotein receptor activity
GO:0005044 scavenger receptor activity
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of CXCL16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸


How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra