Goat anti Mouse CXCL16 antibody, recognizes C-X-C motif Chemokine 16 (CXCL16). Unlike most chemokines, CXCL16 is a membrane bound ligand anchored through transmembrane domains. Although a soluble form of CXCL16 is generated through proteolytic cleavage. Transmembrane CXCL16 is mainly expressed on macrophages, dendritic cells, B cells and various tumor cells. In this form, it acts as an adhesion molecule though the binding of its receptor CXCR6 expressed on other cells including T cells. Transmembrane CXCL16 also binds gram positive and gram negative bacteria, playing a role in innate and adaptive immune responses (Shimaoka et al. 2003).

Soluble CXCL16 has chemoattractant activity, and elicits this response through the receptor CXCR6.There is also mounting evidence suggesting an additional role in tumor progression. With a number of studies suggesting CXCL16 as a biomarker for the evaluation of some tumor type prognoses (Richardsen et al. 2015, Shibata et al. 2020, Gooden et al. 2014).

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		0.5 ug/ml	2.0 ug/ml
Western Blotting		0.1 ug/ml	0.2 ug/ml