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CD184 / CXCR4 antibody

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Goat anti Mouse CD184 / CXCR4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAM67
Datasheet
E P WB
SDS
0.1 mg loader

Goat anti Mouse CD184 / CXCR4 recognises an epitope within the N-terminal (NT) region of mouse CD184, otherwise known as CXCR4 (C-X-C chemokine receptor type 4), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, ubiquitously expressed in blood and tissue cells, which acts as a specific receptor for the chemokine SDF-1 (Stromal cell-derived factor 1).

Interaction of CD184 with SDF-1 mediates the chemotaxis of progenitor and mature blood cells, and is critical for B-lymphopoiesis and myelopoiesis. CD184 is a major co-receptor for infection by T-cell tropic strains of HIV1, and also a primary receptor for some HIV2 isolates.

This antibody is reported as being suitable for use in immunocytochemistry on mouse spleen leucocytes.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Xenopus
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mouse CD184 / CXCR4 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Synthetic peptide YSEEVGSGDYDSNKEPCFRDENVHFNR corresponding to amino acids 14-40 within the N-terminal region of mouse CD184 / CXCR4.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P70658
Entrez Gene
Cxcr4
GO Terms
GO:0001569 patterning of blood vessels
GO:0001667 ameboidal cell migration
GO:0001764 neuron migration
GO:0016021 integral to membrane
GO:0007281 germ cell development
GO:0009887 organ morphogenesis
GO:0007420 brain development
GO:0008045 motor axon guidance
GO:0008354 germ cell migration
GO:0009897 external side of plasma membrane
GO:0016493 C-C chemokine receptor activity
GO:0016494 C-X-C chemokine receptor activity
GO:0030334 regulation of cell migration
GO:0030426 growth cone
GO:0042098 T cell proliferation
Regulatory
For research purposes only

Applications of CD184 / CXCR4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50000
Immunohistology - Paraffin 1/300
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product is suitable for use in indirect ELISA applications.
Histology Positive Control Tissue
Spleen
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

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