Laminin antibody
Rabbit anti Laminin antibody (AHP420) used to evaluate laminin expression on CA1a breast cancer cells using immunofluorescence.
Image caption:
Matrix stiffening induces mechanosensitive signalling promoting a malignant phenotype in breast cancer cells.
Spinning disc confocal immunofluorescent images of CA1a cells on 0.4 kPa and 5 kPa. Scale bar = 20 μm.
From: Göransson, S et al.
Extracellular matrix stiffness-induced breast cancer cell transcriptome resembles the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC).
Biochem Biophys Ras Comms. 2 Mar [Epub ahead of print].
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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P IF ResetRabbit anti Laminin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Laminin
Antibody Quality and Performance Guarantee
| Rabbit anti Laminin antibody recognizes both A (~400 kDa) and B (~220 kDa) chains of laminin. |
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Chicken Duck Birds Expected from Sequence Human Dog Pigeon - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Whole serum - liquid
- Antiserum Preparation
- Antisera to laminin were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified laminin from Engelbreth-Holm-Swarn (EHS) murine sarcoma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
>1/1000 | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/200 | 1/1000 |
| Immunohistology - Paraffin | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human kidney
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
References for Laminin antibody
-
Arnold, S.A. et al. (2012) Losartan Slows Pancreatic Tumor Progression and Extends Survival of SPARC-Null Mice by Abrogating Aberrant TGFβ Activation.
PLoS One. 7(2):e31384. -
Gordon-Weeks, P.R. et al. (1989) Transient expression of laminin immunoreactivity in the developing rat hippocampus.
J Neurocytol. 18 (4): 451-63. -
Gordon-Weeks, P.R. et al. (1992) A study of the expression of laminin in the spinal cord of the frog during development and regeneration.
Exp Physiol. 77 (5): 681-92. -
Abd-Elmaksoud, A. et al. (2009) Comparative expression of laminin and smooth muscle actin in the testis and epididymis of poultry and rabbit.
J Mol Histol. 40: 407-16. -
Au, E. et al. (2007) SPARC from olfactory ensheathing cells stimulates Schwann cells to promote neurite outgrowth and enhances spinal cord repair.
J Neurosci. 27: 7208-21. -
Mueller, S.N. et al. (2007) Viral targeting of fibroblastic reticular cells contributes to immunosuppression and persistence during chronic infection.
Proc Natl Acad Sci U S A. 104: 15430-5. -
Falk, V. et al. (2002) Regulation of matrix metalloproteinases and effect of MMP-inhibition in heart transplant related reperfusion injury.
Eur J Cardiothorac Surg. 22: 53-8. -
Richardson, G.D. et al. (2009) Dynamic expression of Syndecan-1 during hair follicle morphogenesis.
Gene Expr Patterns. 9: 454-60.
View The Latest Product References
-
Miners, J.S. et al. (2008) Angiotensin-converting enzyme (ACE) levels and activity in Alzheimer's disease, and relationship of perivascular ACE-1 to cerebral amyloid angiopathy.
Neuropathol Appl Neurobiol. 34: 181-93. -
Melo, F.A. et al. (2009) Hepatic extracellular matrix alterations in dogs naturally infected with Leishmania (Leishmania) chagasi.
Int J Exp Pathol. 90: 538-48. -
Yeh, T.S. et al. (2014) Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction.
Biomaterials. 35 (1): 174-84. -
Foditsch, E.E. et al. (2016) Skeletal muscle proteins: a new approach to delimitate the time since death.
Int J Legal Med. 130 (2): 433-40. -
Kato, Y. et al. (2015) Targeting Antigen to Clec9A Primes Follicular Th Cell Memory Responses Capable of Robust Recall.
J Immunol. 195 (3): 1006-14. -
Göransson, S. et al. (2023) Extracellular matrix stiffness-induced breast cancer cell transcriptome resembles the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC)
Biochem Biophys Res Comms 2 Mar. [Epub ahead of print]. Pre-proof.
- RRID
- AB_2133777
AHP420
151842
AHP420T
1608
161570
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