WNT-1 antibody
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P ResetRabbit anti Human WNT-1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- WNT-1
Antibody Quality and Performance Guarantee
| Rabbit anti Human WNT-1 antibody recognizes Wnt-1, formerly known as int-1, a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer. Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumor-like virus (Wainwright 1988). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human Wnt-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human Wnt-1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for WNT-1 antibody
-
Brown, A.M. et al. (1987) Identification of protein products encoded by the proto-oncogene int-1.
Mol Cell Biol. 7 (11): 3971-7.
- Synonyms
- WNT-1 Proto-Oncogene Protein
- RRID
- AB_2217026
- UniProt
- P04628
- Entrez Gene
- WNT1
- GO Terms
- GO:0005886 plasma membrane
- GO:0004871 signal transducer activity
- GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
- GO:0005109 frizzled binding
- GO:0005110 frizzled-2 binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0005615 extracellular space
- GO:0005769 early endosome
- GO:0005770 late endosome
- View More GO Terms
- GO:0009611 response to wounding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010592 positive regulation of lamellipodium assembly
- GO:0010812 negative regulation of cell-substrate adhesion
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0021536 diencephalon development
- GO:0021551 central nervous system morphogenesis
- GO:0021588 cerebellum formation
- GO:0021592 fourth ventricle development
- GO:0021797 forebrain anterior/posterior pattern formation
- GO:0022004 midbrain-hindbrain boundary maturation during brain development
- GO:0022408 negative regulation of cell-cell adhesion
- GO:0030901 midbrain development
- GO:0033077 T cell differentiation in thymus
- GO:0035284 brain segmentation
- GO:0042472 inner ear morphogenesis
- GO:0042770 signal transduction in response to DNA damage
- GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
- GO:0044336 canonical Wnt receptor signaling pathway involved in negative regulation of apoptosis
- GO:0045121 membrane raft
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045747 positive regulation of Notch signaling pathway
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0048332 mesoderm morphogenesis
- GO:0048664 neuron fate determination
- GO:0061054 dermatome development
- GO:0070365 hepatocyte differentiation
- GO:0071425 hemopoietic stem cell proliferation
- GO:2000002 negative regulation of DNA damage checkpoint
AHP1287
154743
159279
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