WNT-1 antibody

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Rabbit anti Human WNT-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1287 E P* WB 0.1 mg
Rabbit anti Human WNT-1 antibody recognizes Wnt-1, formerly known as int-1, a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.

Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumor-like virus (Wainwright et al. 1988). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human Wnt-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human Wnt-1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P04628 Related reagents
Entrez Gene
WNT1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0004871 signal transducer activity
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
GO:0005109 frizzled binding
GO:0005110 frizzled-2 binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005769 early endosome
GO:0005770 late endosome
GO:0009611 response to wounding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010592 positive regulation of lamellipodium assembly
GO:0010812 negative regulation of cell-substrate adhesion
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0021536 diencephalon development
GO:0021551 central nervous system morphogenesis
GO:0021588 cerebellum formation
GO:0021592 fourth ventricle development
GO:0021797 forebrain anterior/posterior pattern formation
GO:0022004 midbrain-hindbrain boundary maturation during brain development
GO:0022408 negative regulation of cell-cell adhesion
GO:0030901 midbrain development
GO:0033077 T cell differentiation in thymus
GO:0035284 brain segmentation
GO:0042472 inner ear morphogenesis
GO:0042770 signal transduction in response to DNA damage
GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
GO:0044336 canonical Wnt receptor signaling pathway involved in negative regulation of apoptosis
GO:0045121 membrane raft
GO:0045599 negative regulation of fat cell differentiation
GO:0045747 positive regulation of Notch signaling pathway
GO:0048146 positive regulation of fibroblast proliferation
GO:0048332 mesoderm morphogenesis
GO:0048664 neuron fate determination
GO:0061054 dermatome development
GO:0070365 hepatocyte differentiation
GO:0071425 hemopoietic stem cell proliferation
GO:2000002 negative regulation of DNA damage checkpoint
Regulatory
For research purposes only

Applications of WNT-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for WNT-1 antibody

  1. Brown, A.M. et al. (1987) Identification of protein products encoded by the proto-oncogene int-1.
    Mol Cell Biol. 7 (11): 3971-7.