Rabbit anti Human WNT-1 antibody
recognizes Wnt-1, formerly known as int-1, a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.
Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumor-like virus (Wainwright 1988
). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antisera to human Wnt-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant human Wnt-1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 6 months from date of despatch
- Entrez Gene
- GO Terms
signal transducer activity
Wnt receptor signaling pathway, calcium modulating pathway
proteinaceous extracellular matrix
response to wounding
positive regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of lamellipodium assembly
negative regulation of cell-substrate adhesion
transcription activator activity
central nervous system morphogenesis
fourth ventricle development
forebrain anterior/posterior pattern formation
midbrain-hindbrain boundary maturation during brain development
negative regulation of cell-cell adhesion
T cell differentiation in thymus
inner ear morphogenesis
signal transduction in response to DNA damage
positive regulation of insulin-like growth factor receptor signaling pathway
canonical Wnt receptor signaling pathway involved in negative regulation of apoptosis
negative regulation of fat cell differentiation
positive regulation of Notch signaling pathway
positive regulation of fibroblast proliferation
neuron fate determination
hemopoietic stem cell proliferation
negative regulation of DNA damage checkpoint
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of WNT-1 antibody
|Immunohistology - Paraffin 1
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for WNT-1 antibody
Brown, A.M. et al. (1987) Identification of protein products encoded by the proto-oncogene int-1.
Mol Cell Biol. 7 (11): 3971-7.
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