UBE2K antibody

ISO 9001 Quality Assurance Manufacturing | Print

KO Validated

Anti UBE2K Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti UBE2K Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti UBE2K Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti UBE2K Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with UBE2K antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to UBE2K (molecular weight 22 kDa)

Anti UBE2K Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of UBE2K knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti UBE2K antibody (**VPA00206**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 600 second and (B) 30 second exposure.

Anti UBE2K Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity UBE2K from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Filter by Application:

Rabbit anti UBE2K

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: UBE2K

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00206 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Rabbit anti Human UBE2K antibody recognizes the ubiquitin-conjugating enzyme E2 K, also known as huntingtin interacting protein 2 (HIP-2), ubiquitin carrier protein, ubiquitin-conjugating enzyme E2(25K) and ubiquitin-conjugating enzyme E2-25 kDa.

Encoded by the UBE2K gene, ubiquitin-conjugating enzyme E2 K belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for UBE2K (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE2K antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti UBE2K detects a band of approximately 22 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 2-30 amino acids from the N-terminal region of human UBE2K
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.