UBE2D1 antibody

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Rabbit anti UBE2D1

Rabbit anti human UBE2D1 antibody recognizes ubiquitin-conjugating enzyme E2 D1, also known as UBC4/5 homolog, stimulator of Fe transport, ubiquitin carrier protein D1, or ubiquitin-protein ligase D1. This antibody has been extensively validated for western blotting using whole cell lysates.

UBE2D1 Antibody with Control Lysate

Rabbit anti human UBE2D1 antibody recognizes ubiquitin-conjugating enzyme E2 D1, also known as UBC4/5 homolog, stimulator of Fe transport, ubiquitin carrier protein D1, ubiquitin-conjugating enzyme E2(17)KB 1, ubiquitin-conjugating enzyme E2-17 kDa 1 or ubiquitin-protein ligase D1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00296 WB 100 µl
VPA00296KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human UBE2D1 antibody recognizes UBE2D1, also known as UBC4/5 homolog, stimulator of Fe transport, ubiquitin carrier protein D1, ubiquitin-conjugating enzyme E2 D1, ubiquitin-conjugating enzyme E2-17 kDa 1 and ubiquitin-protein ligase D1.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. The UBE2D1 gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for UBE2D1 (provided by RefSeq, Mar 2011).

Rabbit anti Human UBE2D1 antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 111-140 of human UBE2D1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P51668 Related reagents
Entrez Gene
UBE2D1 Related reagents
GO Terms
GO:0000209 protein polyubiquitination
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0030509 BMP signaling pathway
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0043687 post-translational protein modification
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0070936 protein K48-linked ubiquitination
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UBE2D1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti UBE2D1 detects a band of approximately 17 kDa in HEK293 cell lysate
Western Blotting
Anti UBE2D1 detects a band of approximately 17 kDa in HEK293 cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB