Tubulin Alpha 4A Chain antibody

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Rabbit anti Tubulin Alpha 4A Chain

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00231
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human tubulin alpha 4A chain antibody recognizes tubulin alpha 4A chain, also known as tubulin H2-alpha, tubulin alpha-1 chain, and tubulin, alpha 1 (testis specific).

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. Encoded by the TUBA4A gene, tubulin alpha 4A chain is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for TUBA4A (provided by RefSeq, Jun 2013).

Rabbit anti Human tubulin alpha 4A chain antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti tubulin alpha 4A chain detects a band of approximately 55 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 417-446 amino acids from the C-terminal region of human tubulin alpha 4A chain

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P68366 Related reagents
Entrez Gene
TUBA4A Related reagents
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0002576 platelet degranulation
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0005525 GTP binding
GO:0005874 microtubule
GO:0007018 microtubule-based movement
GO:0030168 platelet activation
GO:0051084 'de novo' posttranslational protein folding
GO:0051258 protein polymerization
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Tubulin Alpha 4A Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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