TSC2 antibody

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Rabbit anti TSC2

TSC2 antibody recognizes tuberin, also known as protein phosphatase 1, regulatory subunit 160, tuberin or tuberous sclerosis 2 protein. This antibody has been extensively validated for western blotting using whole cell lysates.

TSC2 Antibody with Control Lysate

TSC2 antibody recognizes tuberin, also known as protein phosphatase 1, regulatory subunit 160, tuberin or tuberous sclerosis 2 protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00214 WB 100 µl
VPA00214KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human TSC2 antibody recognizes tuberin, also known as protein phosphatase 1, regulatory subunit 160, and tuberous sclerosis 2 protein.

Mutations in the TSC2 gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

Rabbit anti Human Tuberin antibody detects a band of 200 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TSC2 detects a band of approximately 200 kDa in LNCaP cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 1397-1426 amino acids from the central region of human TSC2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg LNCaP lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P49815 Related reagents
Entrez Gene
TSC2 Related reagents
GO Terms
GO:0001843 neural tube closure
GO:0006606 protein import into nucleus
GO:0005096 GTPase activator activity
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0006897 endocytosis
GO:0007050 cell cycle arrest
GO:0007507 heart development
GO:0008286 insulin receptor signaling pathway
GO:0014067 negative regulation of phosphatidylinositol 3-kinase cascade
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030100 regulation of endocytosis
GO:0033596 TSC1-TSC2 complex
GO:0042803 protein homodimerization activity
GO:0043491 protein kinase B signaling cascade
GO:0043547 positive regulation of GTPase activity
GO:0046626 regulation of insulin receptor signaling pathway
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0048471 perinuclear region of cytoplasm
GO:0050918 positive chemotaxis
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0051898 negative regulation of protein kinase B signaling cascade
GO:0071109 superior temporal gyrus development
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of TSC2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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