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TSC2 antibody

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Anti TSC2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti TSC2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti TSC2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti TSC2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with TSC2 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 17 second exposure. Arrow points to TSC2 (molecular weight 200 kDa)
Anti TSC2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of TSC2 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Rabbit anti TSC2 antibody (VPA00214) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 300 second and (B) 30 second exposure.
Anti TSC2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of TSC2 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Rabbit anti TSC2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
TSC2

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Product Code Applications Pack Size List Price Your Price Qty
VPA00214
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Rabbit anti Human TSC2 antibody recognizes tuberin, also known as protein phosphatase 1, regulatory subunit 160, and tuberous sclerosis 2 protein.

Mutations in the TSC2 gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

Rabbit anti Human Tuberin antibody detects a band of 200 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti TSC2 detects a band of approximately 200 kDa in LNCaP cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide between 1397-1426 amino acids from the central region of human TSC2
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P49815
Entrez Gene
TSC2
GO Terms
GO:0001843 neural tube closure
GO:0006606 protein import into nucleus
GO:0005096 GTPase activator activity
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0006897 endocytosis
GO:0007050 cell cycle arrest
GO:0007507 heart development
GO:0008286 insulin receptor signaling pathway
GO:0014067 negative regulation of phosphatidylinositol 3-kinase cascade
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030100 regulation of endocytosis
GO:0033596 TSC1-TSC2 complex
GO:0042803 protein homodimerization activity
GO:0043491 protein kinase B signaling cascade
GO:0043547 positive regulation of GTPase activity
GO:0046626 regulation of insulin receptor signaling pathway
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0048471 perinuclear region of cytoplasm
GO:0050918 positive chemotaxis
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0051898 negative regulation of protein kinase B signaling cascade
GO:0071109 superior temporal gyrus development

VPA00214

151204

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