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Transferrin antibody

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Sheep anti Human Transferrin:FITC

Sheep anti Human Transferrin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
9100-0004F
Datasheet
IF
SDS
1 ml

9100-0004
Datasheet
E
SDS
1 ml

Sheep anti Human transferrin antibody recognizes human transferrin, an approximately 80 kDa blood plasma glycoprotein synthesised by the liver, which contains two specific high affinity iron (Fe3+) binding sites and is responsible for the transport and supply of an exchangeable pool of iron, through binding to cell surface transferrin receptors.

Transferrin is the primary blood iron transport protein and under normal conditions, approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.

Sheep anti Human transferrin antibody shows minimal cross reactivity with related serum proteins.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human transferrin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Pure human transferrin prepared from pooled normal human serum.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P02787
Entrez Gene
TF
GO Terms
GO:0002576 platelet degranulation
GO:0005576 extracellular region
GO:0005769 early endosome
GO:0005770 late endosome
GO:0030141 stored secretory granule
GO:0030139 endocytic vesicle
GO:0005905 coated pit
GO:0006879 cellular iron ion homeostasis
GO:0008199 ferric iron binding
GO:0009925 basal plasma membrane
GO:0010008 endosome membrane
GO:0016324 apical plasma membrane
GO:0030168 platelet activation
GO:0033572 transferrin transport
GO:0048471 perinuclear region of cytoplasm
GO:0055037 recycling endosome
GO:0055085 transmembrane transport
Regulatory
For research purposes only

Applications of Transferrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Product Specific References

References for Transferrin antibody

  1. Rouault, T.A. (2003) How mammals acquire and distribute iron needed for oxygen-based metabolism.
    PLoS Biol. 1 (3): E79.
  2. Olkhov, R. and Shaw, A.M. (2014) Growth kinetics of gold nanoparticles on silica/graphene surfaces for multiplex biological immunoassays.
    RSC Adv., 2014,4, 31678-84.

Further Reading

  1. Giannetti, A.M. et al. (2003) Mechanism for multiple ligand recognition by the human transferrin receptor.
    PLoS Biol. 1 (3): E51.

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