Transferrin antibody
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Sheep anti Human transferrin antibody recognizes human transferrin, an approximately 80 kDa blood plasma glycoprotein synthesised by the liver, which contains two specific high affinity iron (Fe3+) binding sites and is responsible for the transport and supply of an exchangeable pool of iron, through binding to cell surface transferrin receptors.
Transferrin is the primary blood iron transport protein and under normal conditions, approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.
Sheep anti Human transferrin antibody shows minimal cross reactivity with related serum proteins.
Transferrin is the primary blood iron transport protein and under normal conditions, approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.
Sheep anti Human transferrin antibody shows minimal cross reactivity with related serum proteins.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human transferrin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Pure human transferrin prepared from pooled normal human serum.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P02787 Related reagents
- Entrez Gene
- TF Related reagents
- GO Terms
- GO:0002576 platelet degranulation
- GO:0005576 extracellular region
- GO:0005769 early endosome
- GO:0005770 late endosome
- GO:0030141 stored secretory granule
- GO:0030139 endocytic vesicle
- GO:0005905 coated pit
- GO:0006879 cellular iron ion homeostasis
- GO:0008199 ferric iron binding
- GO:0009925 basal plasma membrane
- GO:0010008 endosome membrane
- GO:0016324 apical plasma membrane
- GO:0030168 platelet activation
- GO:0033572 transferrin transport
- GO:0048471 perinuclear region of cytoplasm
- GO:0055037 recycling endosome
- GO:0055085 transmembrane transport
- Regulatory
- For research purposes only
Applications of Transferrin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | 1.5 ml | C E F WB | ||
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:DyLight®549 | STAR88D549GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:DyLight®649 | STAR88D649GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:FITC | STAR88F | 1 mg | C | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
Product Specific References
References for Transferrin antibody
-
Rouault, T.A. (2003) How mammals acquire and distribute iron needed for oxygen-based metabolism.
PLoS Biol. 1 (3): E79. -
Olkhov, R. and Shaw, A.M. (2014) Growth kinetics of gold nanoparticles on silica/graphene surfaces for multiplex biological immunoassays.
RSC Adv., 2014,4, 31678-84.
Further Reading
-
Giannetti, A.M. et al. (2003) Mechanism for multiple ligand recognition by the human transferrin receptor.
PLoS Biol. 1 (3): E51.
