Tissue Transglutaminase antibody

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Rabbit anti Tissue Transglutaminase

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00217
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human tissue transglutaminase antibody recognizes tissue transglutaminase, also known as protein-glutamine gamma-glutamyltransferase 2, C polypeptide, TG(C), TGase C, TGase H, TGase-2, TGase-H, epididymis secretory protein Li 45, transglutaminase C, transglutaminase H and transglutaminase-2.

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. Encoded by the TGM2 gene, tissue transglutaminase acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. It is also the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for TGM2 (provided by RefSeq, Jul 2008).

Rabbit anti Human tissue transglutaminase antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti TGM2 detects a band of approximately 78 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human tissue transglutaminase

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P21980
Entrez Gene
TGM2
GO Terms
GO:0003810 protein-glutamine gamma-glutamyltransferase activity
GO:0046872 metal ion binding
GO:0008415 acyltransferase activity
GO:0043277 apoptotic cell clearance
GO:0045785 positive regulation of cell adhesion
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Tissue Transglutaminase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml