Thymopoetin antibody
Rabbit anti Thymopoetin
Thymopoietin antibody recognizes lamina-associated polypeptide 2, isoform alpha, also known as TMPO, LEM domain containing 4, TP alpha, TP beta/gamma, thymopoietin-related peptide isoform alpha or thymopoietin-related peptide isoforms beta/gamma.
Thymopoetin Antibody with Control Lysate
Thymopoietin antibody recognizes lamina-associated polypeptide 2, isoform alpha, also known as TMPO, LEM domain containing 4, TP alpha, TP beta/gamma, thymopoietin-related peptide isoform alpha or thymopoietin-related peptide isoforms beta/gamma.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00229 | WB | 100 µl | ||||
| VPA00229KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human thymopoietin antibody recognizes the beta isoform of thymopoietin, also known as lamina-associated polypeptide 2, isoform beta, TP beta and thymopoietin-related peptide isoform beta.
Encoded by the TMPO gene, thymopoietins reside in the nucleus and may play a role in the assembly of the nuclear lamina, helping to maintain the structural organization of the nuclear envelope. Mutations in TMPO are associated with dilated cardiomyopathy and this gene has been found up regulated in several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for TMPO (provided by RefSeq, May 2010).
Rabbit anti Human thymopoietin antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the TMPO gene, thymopoietins reside in the nucleus and may play a role in the assembly of the nuclear lamina, helping to maintain the structural organization of the nuclear envelope. Mutations in TMPO are associated with dilated cardiomyopathy and this gene has been found up regulated in several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for TMPO (provided by RefSeq, May 2010).
Rabbit anti Human thymopoietin antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti thymopoetin detects a band of approximately 51 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 243-269 amino acids from the central region of human thymopoetin
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P42167 Related reagents
- Entrez Gene
- TMPO Related reagents
- GO Terms
- GO:0003677 DNA binding
- GO:0005521 lamin binding
- GO:0016021 integral to membrane
- GO:0005637 nuclear inner membrane
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Thymopoetin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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