TAP1 antibody

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Rabbit anti Human TAP1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1248 E F IP WB 50 µg
Rabbit anti Human TAP1 antibody detects an epitope within the C-terminal region of human TAP1, also known as transporter associated with antigen processing 1. TAP1 is an ~81 kDa membrane-associated protein which is a member of the ATP-binding cassette (ABC) transporter superfamily and together with the homologous subunit TAP2 forms the functional TAP complex.

TAP1 is involved in the transport of antigenic peptides from the cytosol into the endoplasmic reticulum, where they associate with major histocompatibility complex (MHC) class I molecules. These complexes are presented at the cell surface to T-lymphocytes, which identify and remove infected or malignantly transformed cells.

Rabbit anti Human TAP1 antibody ( AHP1248) does not cross react with human TAP2.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to TAP1 (C-terminal) were raised by repeated immunisations of rabbits with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
TAP1 peptide corresponding to the C-terminal of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q03518 Related reagents
Entrez Gene
TAP1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0005829 cytosol
GO:0042605 peptide antigen binding
GO:0005524 ATP binding
GO:0015421 oligopeptide-transporting ATPase activity
GO:0019060 intracellular transport of viral proteins in host cell
GO:0019885 antigen processing and presentation of endogenous peptide antigen via MHC class I
GO:0042288 MHC class I protein binding
GO:0042803 protein homodimerization activity
GO:0042825 TAP complex
GO:0043531 ADP binding
GO:0046967 cytosol to ER transport
GO:0046978 TAP1 binding
GO:0046979 TAP2 binding
GO:0046980 tapasin binding
Regulatory
For research purposes only

Applications of TAP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10000
Flow Cytometry
Immunoprecipitation
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1248 detects a band of approximately 72kDa

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*