SUMO-1 antibody

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Rabbit anti SUMO-1 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1184
Datasheet
C WB
SDS
0.1 mg loader

Rabbit anti Human SUMO-1 antibody recognizes the N-terminal (NT) region of small ubiquitin-related modifier-1 (SUMO-1). SUMO-1 is an ~11 kDa protein which structurally related to ubiquitin, and is added to proteins as a form of post-translational modification known as sumoylation. Sumoylation can be cell cycle regulated, stimulated upon stress or induced by DNA damage, and binding elicits individual effects for each protein. Effects include changes to protein stability, nuclear-cytosolic transport, and transcriptional regulation (mostly transcriptional repression).

Alternate transcriptional splice variants encoding different isoforms have been characterized.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human SUMO-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
14 amino acid peptide sequence near the amino terminus of human SUMO-1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P63165
Entrez Gene
SUMO1
GO Terms
GO:0005643 nuclear pore
GO:0005737 cytoplasm
GO:0006281 DNA repair
GO:0016481 negative regulation of transcription
GO:0016607 nuclear speck
GO:0016925 protein sumoylation
GO:0031334 positive regulation of protein complex assembly
GO:0031625 ubiquitin protein ligase binding
GO:0031965 nuclear membrane
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0032880 regulation of protein localization
GO:0043433 negative regulation of transcription factor activity
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
Regulatory
For research purposes only

Applications of SUMO-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 0.5 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1184 detects a band of approximately 15kDa in HL-60 cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Product Specific References

Further Reading

  1. Melchior, F. & Hengst, L. (2002) SUMO-1 and p53.
    Cell Cycle. 1 (4): 245-9.
  2. Verger, A. et al. (2003) Modification with SUMO. A role in transcriptional regulation.
    EMBO Rep. 4 (2): 137-42.
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