SUMO-1 antibody
Rabbit anti SUMO-1 (N-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- SUMO-1
- Region
- (N-TERMINAL)
Antibody Quality and Performance Guarantee
| Rabbit anti Human SUMO-1 antibody recognizes the N-terminal (NT) region of small ubiquitin-related modifier-1 (SUMO-1). SUMO-1 is an ~11 kDa protein which structurally related to ubiquitin, and is added to proteins as a form of post-translational modification known as sumoylation. Sumoylation can be cell cycle regulated, stimulated upon stress or induced by DNA damage, and binding elicits individual effects for each protein. Effects include changes to protein stability, nuclear-cytosolic transport, and transcriptional regulation (mostly transcriptional repression). Alternate transcriptional splice variants encoding different isoforms have been characterized. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human SUMO-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- 14 amino acid peptide sequence near the amino terminus of human SUMO-1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Western Blotting | |
0.5 | 1.0ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1184 detects a band of approximately 15kDa in HL-60 cell lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
Further Reading
-
Melchior, F. & Hengst, L. (2002) SUMO-1 and p53.
Cell Cycle. 1 (4): 245-9. -
Verger, A. et al. (2003) Modification with SUMO. A role in transcriptional regulation.
EMBO Rep. 4 (2): 137-42.
- Synonyms
- Small UBIQUITIN-RELATED MODIFIER-1
- RRID
- AB_2198094
- UniProt
- P63165
- Entrez Gene
- SUMO1
- GO Terms
- GO:0005643 nuclear pore
- GO:0005737 cytoplasm
- GO:0006281 DNA repair
- GO:0016481 negative regulation of transcription
- GO:0016607 nuclear speck
- GO:0016925 protein sumoylation
- GO:0031334 positive regulation of protein complex assembly
- GO:0031625 ubiquitin protein ligase binding
- GO:0031965 nuclear membrane
- View More GO Terms
- GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
- GO:0032880 regulation of protein localization
- GO:0043433 negative regulation of transcription factor activity
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060334 regulation of interferon-gamma-mediated signaling pathway
AHP1184
152913
163824
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