STAT1 (pTyr701) antibody
Rabbit anti STAT1 (pTyr701)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- STAT1 (pTyr701)
Antibody Quality and Performance Guarantee
| Rabbit anti STAT1 (pTyr701) antibody recognizes STAT1, also known as transcription factor ISGF-3 components p91/p84, when phosphorylated at tyrosine 701. STAT1 is part of the transcription factor STAT family of proteins. STAT proteins form homo- or hetero-dimers once phosphorylated by receptor associated kinases. Following dimerization STAT proteins then translocate to the cell nucleus. Phosphorylation at tyrosine 701 induces dimerization and activates the transcriptional activity of STAT1 (Wenta et al. 2008). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to STAT1 (pTyr701) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% ProClin 300
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 701 of human STAT1
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Wenta, N. et al. (2008) Tyrosine phosphorylation regulates the partitioning of STAT1 between different dimer conformations.
Proc Natl Acad Sci U S A. 105 (27): 9238-43.
AHP2683
141637
156572
161453
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