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STAT1 (pTyr701) antibody

Rabbit anti STAT1 (pTyr701)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
STAT1 (pTyr701)

Product Code Applications Pack Size List Price Your Price Qty
AHP2683
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µl loader
List Price Your Price
loader

Rabbit anti STAT1 (pTyr701) antibody recognizes STAT1, also known as transcription factor ISGF-3 components p91/p84, when phosphorylated at tyrosine 701. STAT1 is part of the transcription factor STAT family of proteins. STAT proteins form homo- or hetero-dimers once phosphorylated by receptor associated kinases. Following dimerization STAT proteins then translocate to the cell nucleus. Phosphorylation at tyrosine 701 induces dimerization and activates the transcriptional activity of STAT1 (Wenta et al. 2008).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to STAT1 (pTyr701) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% ProClin 300
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 701 of human STAT1
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Wenta, N. et al. (2008) Tyrosine phosphorylation regulates the partitioning of STAT1 between different dimer conformations.
    Proc Natl Acad Sci U S A. 105 (27): 9238-43.

UniProt
P42224
Entrez Gene
STAT1

AHP2683

141637 156572 161453

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