STAT1 (pSer727) antibody
Rabbit anti STAT1 (pSer727)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- STAT1 (pSer727)
Rabbit anti STAT1 (pSer727) antibody recognizes signal transducer and activator of transcription 1-alpha/beta (STAT1) also known as transcription factor ISGF-3 components p91/p84, when phosphorylated at serine 727. STAT1 is part of the transcription factor STAT family of proteins. STAT proteins form homo- or hetero-dimers once phosphorylated by receptor associated kinases. Following dimerization STAT proteins then translocate to the cell nucleus. Phosphorylation of STAT1 at serine 727 occurs via p38 MAPK (MAPK14) pathways (Ramsauer et al. 2002). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to STAT1 (pSer727) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% ProClin 300
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 727 of human STAT1
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin | 1/50 | 1/100 | |
Western Blotting | 1/500 | 1/2000 |
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Further Reading
-
Ramsauer, K. et al. (2002) p38 MAPK enhances STAT1-dependent transcription independently of Ser-727 phosphorylation.
Proc Natl Acad Sci U S A. 99 (20): 12859-64.
AHP2682
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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