SMAD3 antibody

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Rabbit anti SMAD3

SMAD3 antibody recognizes mothers against decapentaplegic homolog 3, also known as MAD homolog 3, MAD, SMA- and MAD-related protein 3, SMAD, mothers against DPP homolog 3, hMAD-3, hSMAD3, mad homolog JV15-2 or Mad3.

SMAD3 Antibody with Control Lysate

SMAD3 antibody recognizes mothers against decapentaplegic homolog 3, also known as MAD homolog 3, MAD, SMA- and MAD-related protein 3, SMAD, mothers against DPP homolog 3, hMAD-3, hSMAD3, mad homolog JV15-2 or Mad3.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00166 WB 100 µl
VPA00166KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human SMAD3 antibody recognizes SMAD3, also known as MAD homolog 3, Mad3, mothers against DPP homolog 3, hMAD-3, JV15-2 SMAD family member 3, Smad3 and hSMAD3.

SMAD3 belongs to the SMAD family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis (provided by RefSeq, Apr 2009).

Rabbit anti Human SMAD3 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to an Internal region of human SMAD3 surrounding amino acid residue 179

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P84022 Related reagents
Entrez Gene
SMAD3 Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of SMAD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti SMAD3 detects a band of approximately 52 kDa in HEK293 cell lysates.
Western Blotting
Anti SMAD3 detects a band of approximately 52 kDa in HEK293 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB