SMAD3 antibody

Rabbit anti SMAD3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
SMAD3

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VPA00166
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Rabbit anti Human SMAD3 antibody recognizes SMAD3, also known as MAD homolog 3, Mad3, mothers against DPP homolog 3, hMAD-3, JV15-2 SMAD family member 3, Smad3 and hSMAD3.

SMAD3 belongs to the SMAD family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis (provided by RefSeq, Apr 2009).

Rabbit anti Human SMAD3 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti SMAD3 detects a band of approximately 52 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to an Internal region of human SMAD3 surrounding amino acid residue 179
Approx. Protein Concentrations
IgG concentration 0.17 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P84022
Entrez Gene
SMAD3
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products