Slug antibody
Rabbit anti Slug antibody detects Slug, a member of the Snail family of zinc finger transcription factors, whose expression is induced by FGF, BMP, and TGF-β. Slug is a ~29 kDa transcriptional repressor, which functions by binding to E-box motifs and inhibiting gene expression by recruiting histone deacetylase-1 (HDAC-1).
Slug controls the expression of a wide variety of genes including TP53, BRCA2, PUM and CDH1, and has important roles in apoptosis and epithelial-mesenchymal transition. It is also thought to function in neural crest development.
Slug expression in breast, oesophageal, and colorectal carcinomas has been correlated with poor prognosis for survival. Rabbit anti Slug antibody is not cross-reactive with Snail.
Slug controls the expression of a wide variety of genes including TP53, BRCA2, PUM and CDH1, and has important roles in apoptosis and epithelial-mesenchymal transition. It is also thought to function in neural crest development.
Slug expression in breast, oesophageal, and colorectal carcinomas has been correlated with poor prognosis for survival. Rabbit anti Slug antibody is not cross-reactive with Snail.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human Slug were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- 13 amino acid peptide sequence near the centre of human Slug.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O43623
- Entrez Gene
- SNAI2
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0001649 osteoblast differentiation
- GO:0003677 DNA binding
- GO:0005634 nucleus
- GO:0006350 transcription
- GO:0007275 multicellular organismal development
- GO:0007499 ectoderm and mesoderm interaction
- GO:0008270 zinc ion binding
- GO:0060070 canonical Wnt receptor signaling pathway
- Regulatory
- For research purposes only
Applications of Slug antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 0.5 | 2.0ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1186 detects a band of approximately 30kDa in 293 cell lysate.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Tripathi, M.K. et al. (2005) Regulation of BRCA2 gene expression by the SLUG repressor protein in human breast cells.
J Biol Chem. 280 (17): 17163-71.
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