Rabbit anti Slug antibody detects Slug, a member of the Snail family of zinc finger transcription factors, whose expression is induced by FGF, BMP, and TGF-β. Slug is a ~29 kDa transcriptional repressor, which functions by binding to E-box motifs and inhibiting gene expression by recruiting histone deacetylase-1 (HDAC-1).
Slug controls the expression of a wide variety of genes including TP53, BRCA2, PUM and CDH1, and has important roles in apoptosis and epithelial-mesenchymal transition. It is also thought to function in neural crest development.
Slug expression in breast, oesophageal, and colorectal carcinomas has been correlated with poor prognosis for survival. Rabbit anti Slug antibody is not cross-reactive with Snail.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human Slug were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 13 amino acid peptide sequence near the centre of human Slug.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of transcription from RNA polymerase II promoter
multicellular organismal development
ectoderm and mesoderm interaction
zinc ion binding
canonical Wnt receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Slug antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1186 detects a band of approximately 30kDa in 293 cell lysate.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Tripathi, M.K. et al. (2005) Regulation of BRCA2 gene expression by the SLUG repressor protein in human breast cells.
J Biol Chem. 280 (17): 17163-71.
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