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SIRT1 antibody

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Western Blot Validated
Anti SIRT1 Antibody (PrecisionAb™ Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with SIRT1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to SIRT1 (molecular weight 120 kDa)

Rabbit anti SIRT1

Rabbit anti human SIRT1 antibody recognizes NAD-dependent protein deacetylase sirtuin-1, also known as SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, regulatory protein SIR2 homolog 1, sir2-like 1 or sirtuin type 1. This antibody has been validated for western blotting using whole cell lysates.

SIRT1 Antibody with Control Lysate

Rabbit anti human SIRT1 antibody recognizes NAD-dependent protein deacetylase sirtuin-1, also known as SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, regulatory protein SIR2 homolog 1, sir2-like 1 or sirtuin type 1. This antibody has been extensively validated for western blotting.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00294 WB 100 µl
VPA00294KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human SIRT1 antibody recognizes SIRT1, also known as NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, regulatory protein SIR2 homolog 1 and sirtuin type 1.

The SIRT1 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by SIRT1 is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants (provided by RefSeq, Dec 2008).

Rabbit anti Human SIRT1 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti SIRT1 detects a band of approximately 120 kDa in NIH/3T3 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 587-621 of human SIRT1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg NIH/3T3 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q96EB6 Related reagents
Entrez Gene
SIRT1 Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0000183 chromatin silencing at rDNA
GO:0002039 p53 binding
GO:0003714 transcription corepressor activity
GO:0042393 histone binding
GO:0006915 apoptosis
GO:0005637 nuclear inner membrane
GO:0005737 cytoplasm
GO:0005677 chromatin silencing complex
GO:0005719 nuclear euchromatin
GO:0005720 nuclear heterochromatin
GO:0005730 nucleolus
GO:0006281 DNA repair
GO:0006343 establishment of chromatin silencing
GO:0006344 maintenance of chromatin silencing
GO:0006364 rRNA processing
GO:0006642 triglyceride mobilization
GO:0007517 muscle organ development
GO:0007569 cell aging
GO:0008022 protein C-terminus binding
GO:0008270 zinc ion binding
GO:0042802 identical protein binding
GO:0009267 cellular response to starvation
GO:0042981 regulation of apoptosis
GO:0016564 transcription repressor activity
GO:0016575 histone deacetylation
GO:0016605 PML body
GO:0018394 peptidyl-lysine acetylation
GO:0031937 positive regulation of chromatin silencing
GO:0032071 regulation of endodeoxyribonuclease activity
GO:0033158 regulation of protein import into nucleus, translocation
GO:0033553 rDNA heterochromatin
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0034983 peptidyl-lysine deacetylation
GO:0042127 regulation of cell proliferation
GO:0043398 HLH domain binding
GO:0043425 bHLH transcription factor binding
GO:0043433 negative regulation of transcription factor activity
GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0044419 interspecies interaction between organisms
GO:0045599 negative regulation of fat cell differentiation
GO:0045739 positive regulation of DNA repair
GO:0045768 positive regulation of anti-apoptosis
GO:0046969 NAD-dependent histone deacetylase activity (H3-K9 specific)
GO:0050872 white fat cell differentiation
GO:0051097 negative regulation of helicase activity
GO:0070403 NAD+ binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SIRT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
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