SIRT1 antibody

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Anti SIRT1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SIRT1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to SIRT1 (molecular weight 120 kDa)

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Rabbit anti SIRT1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: SIRT1

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VPA00294 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human SIRT1 antibody recognizes SIRT1, also known as NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, regulatory protein SIR2 homolog 1 and sirtuin type 1.

The SIRT1 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity (Carafa et al. 2016). The protein encoded by SIRT1 is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants (provided by RefSeq, Dec 2008).

Rabbit anti Human SIRT1 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SIRT1 detects a band of approximately 120 kDa in NIH/3T3 cell lysate.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH-conjugated synthetic peptide corresponding to aa 587-621 of human SIRT1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q96EB6
Entrez Gene: SIRT1
GO Terms: GO:0006260 DNA replication; GO:0000122 negative regulation of transcription from RNA polymerase II promoter; GO:0000183 chromatin silencing at rDNA; GO:0002039 p53 binding; GO:0003714 transcription corepressor activity; GO:0042393 histone binding; GO:0006915 apoptosis; GO:0005637 nuclear inner membrane; GO:0005737 cytoplasm; View More GO Terms; GO:0005677 chromatin silencing complex; GO:0005719 nuclear euchromatin; GO:0005720 nuclear heterochromatin; GO:0005730 nucleolus; GO:0006281 DNA repair; GO:0006343 establishment of chromatin silencing; GO:0006344 maintenance of chromatin silencing; GO:0006364 rRNA processing; GO:0006642 triglyceride mobilization; GO:0007517 muscle organ development; GO:0007569 cell aging; GO:0008022 protein C-terminus binding; GO:0008270 zinc ion binding; GO:0042802 identical protein binding; GO:0009267 cellular response to starvation; GO:0042981 regulation of apoptosis; GO:0016564 transcription repressor activity; GO:0016575 histone deacetylation; GO:0016605 PML body; GO:0018394 peptidyl-lysine acetylation; GO:0031937 positive regulation of chromatin silencing; GO:0032071 regulation of endodeoxyribonuclease activity; GO:0033158 regulation of protein import into nucleus, translocation; GO:0033553 rDNA heterochromatin; GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor; GO:0034983 peptidyl-lysine deacetylation; GO:0042127 regulation of cell proliferation; GO:0043398 HLH domain binding; GO:0043425 bHLH transcription factor binding; GO:0043433 negative regulation of transcription factor activity; GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator; GO:0044419 interspecies interaction between organisms; GO:0045599 negative regulation of fat cell differentiation; GO:0045739 positive regulation of DNA repair; GO:0045768 positive regulation of anti-apoptosis; GO:0046969 NAD-dependent histone deacetylase activity (H3-K9 specific); GO:0050872 white fat cell differentiation; GO:0051097 negative regulation of helicase activity; GO:0070403 NAD+ binding