SIRT1 antibody
Rabbit anti Human SIRT1 antibody recognizes SIRT1, also known as NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, regulatory protein SIR2 homolog 1 and sirtuin type 1.
The SIRT1 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by SIRT1 is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants (provided by RefSeq, Dec 2008).
Rabbit anti Human SIRT1 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The SIRT1 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by SIRT1 is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants (provided by RefSeq, Dec 2008).
Rabbit anti Human SIRT1 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti SIRT1 detects a band of approximately 120 kDa in NIH/3T3 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 587-621 of human SIRT1
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q96EB6
- Entrez Gene
- SIRT1
- GO Terms
- GO:0006260 DNA replication
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0000183 chromatin silencing at rDNA
- GO:0002039 p53 binding
- GO:0003714 transcription corepressor activity
- GO:0042393 histone binding
- GO:0006915 apoptosis
- GO:0005637 nuclear inner membrane
- GO:0005737 cytoplasm
- GO:0005677 chromatin silencing complex
- GO:0005719 nuclear euchromatin
- GO:0005720 nuclear heterochromatin
- GO:0005730 nucleolus
- GO:0006281 DNA repair
- GO:0006343 establishment of chromatin silencing
- GO:0006344 maintenance of chromatin silencing
- GO:0006364 rRNA processing
- GO:0006642 triglyceride mobilization
- GO:0007517 muscle organ development
- GO:0007569 cell aging
- GO:0008022 protein C-terminus binding
- GO:0008270 zinc ion binding
- GO:0042802 identical protein binding
- GO:0009267 cellular response to starvation
- GO:0042981 regulation of apoptosis
- GO:0016564 transcription repressor activity
- GO:0016575 histone deacetylation
- GO:0016605 PML body
- GO:0018394 peptidyl-lysine acetylation
- GO:0031937 positive regulation of chromatin silencing
- GO:0032071 regulation of endodeoxyribonuclease activity
- GO:0033158 regulation of protein import into nucleus, translocation
- GO:0033553 rDNA heterochromatin
- GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
- GO:0034983 peptidyl-lysine deacetylation
- GO:0042127 regulation of cell proliferation
- GO:0043398 HLH domain binding
- GO:0043425 bHLH transcription factor binding
- GO:0043433 negative regulation of transcription factor activity
- GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
- GO:0044419 interspecies interaction between organisms
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045739 positive regulation of DNA repair
- GO:0045768 positive regulation of anti-apoptosis
- GO:0046969 NAD-dependent histone deacetylase activity (H3-K9 specific)
- GO:0050872 white fat cell differentiation
- GO:0051097 negative regulation of helicase activity
- GO:0070403 NAD+ binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of SIRT1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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