sIL-4 Receptor Subunit Alpha antibody

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Rabbit anti Human Soluble Interleukin-4 Receptor Subunit Alpha:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2809Z
Datasheet
E WB
SDS
0.1 mg loader

Rabbit anti Human sIL-4 receptor alpha antibody, recognizes soluble interleukin-4 receptor subunit alpha (sIL-4RA), also known as IL-4 binding protein.

sIL-4RA is the naturally occurring soluble form of IL-4RA, a type I receptor which is a functional receptor of IL-4 and IL-13 (Henderson et al. 2000). sIL-4RA is present in biological fluids and contains only the extracellular portion of IL-4RA (Andrews et al. 2006). It is produced by proteolytic shedding of the membrane bound form or through alternative splicing of the gene. Like IL-4RA, the soluble form is able to bind IL-4, which effectively inhibits the biological function of IL-4 (Silvestri et al. 2006).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Antiserum Preparation
Produced from sera of rabbits immunized with recombinant human soluble interleukin-4 receptor subunit alpha and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
CHO cell line derived recombinant soluble interleukin-4 receptor subunit alpha (sIL-4RA) corresponding to 209 amino acid residues of the extracellular domain of IL-4RA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Endotoxin Level
< 0.1 ng/μg of protein (< 1 EU/μg)

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P24394
Entrez Gene
IL4R
Regulatory
For research purposes only

Applications of sIL-4 Receptor Subunit Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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