sIL-4 Receptor Subunit Alpha antibody
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Rabbit anti Human Soluble Interleukin-4 Receptor Subunit Alpha:Preservative Free
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- sIL-4 Receptor Subunit Alpha
Antibody Quality and Performance Guarantee
| Rabbit anti Human sIL-4 receptor alpha antibody, recognizes soluble interleukin-4 receptor subunit alpha (sIL-4RA), also known as IL-4 binding protein. sIL-4RA is the naturally occurring soluble form of IL-4RA, a type I receptor which is a functional receptor of IL-4 and IL-13 (Henderson et al. 2000). sIL-4RA is present in biological fluids and contains only the extracellular portion of IL-4RA (Andrews et al. 2006). It is produced by proteolytic shedding of the membrane bound form or through alternative splicing of the gene. Like IL-4RA, the soluble form is able to bind IL-4, which effectively inhibits the biological function of IL-4 (Silvestri et al. 2006). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water
- Antiserum Preparation
- Produced from sera of rabbits immunized with recombinant human soluble interleukin-4 receptor subunit alpha and purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- CHO cell line derived recombinant soluble interleukin-4 receptor subunit alpha (sIL-4RA) corresponding to 209 amino acid residues of the extracellular domain of IL-4RA
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Endotoxin Level
- < 0.1 ng/μg of protein (< 1 EU/μg)
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 ug/ml | 2.0 ug/ml |
| Western Blotting | |
0.1 ug/ml | 0.2 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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AHP2809Z
156483
165497
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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