SAFB antibody

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Anti SAFB Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with SAFB antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000, STAR208P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to SAFB (molecular weight 150 kDa)

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Rabbit anti SAFB

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: SAFB

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VPA00251 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human SAFB antibody recognizes the scaffold attachment factor B1, also known as HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein, SAB-B1 and SAF-B.

Encoded by the SAFB gene, scaffold attachment factor B1 encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. Scaffold attachment factor B1 is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. SAFB is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for SAFB (provided by RefSeq, Jan 2011).

Rabbit anti Human SAFB antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti SAFB detects a band of approximately 150 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 65-94 amino acids from the N-terminal region of human SAFB
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q15424
Entrez Gene: SAFB
GO Terms: GO:0000166 nucleotide binding; GO:0005515 protein binding; GO:0003690 double-stranded DNA binding; GO:0003723 RNA binding; GO:0005634 nucleus; GO:0006325 chromatin organization; GO:0006350 transcription; GO:0045449 regulation of transcription