SAFB antibody

Rabbit anti SAFB

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
SAFB

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VPA00251
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Rabbit anti Human SAFB antibody recognizes the scaffold attachment factor B1, also known as HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein, SAB-B1 and SAF-B.

Encoded by the SAFB gene, scaffold attachment factor B1 encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. Scaffold attachment factor B1 is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. SAFB is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for SAFB (provided by RefSeq, Jan 2011).

Rabbit anti Human SAFB antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti SAFB detects a band of approximately 150 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide between 65-94 amino acids from the N-terminal region of human SAFB
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q15424
Entrez Gene
SAFB
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0003690 double-stranded DNA binding
GO:0003723 RNA binding
GO:0005634 nucleus
GO:0006325 chromatin organization
GO:0006350 transcription
GO:0045449 regulation of transcription
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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