RPS17 antibody
Rabbit anti RPS17L
Rabbit anti Human RPS17 antibody recognizes 40S ribosomal protein S17, also known as S17, DBA4, RPS17L, RPS17L1 or RPS17L2. This antibody has been extensively validated for western blotting using whole cell lysates.
RPS17L Antibody with Control Lysate
Rabbit anti Human RPS17 antibody recognizes 40S ribosomal protein S17, also known as S17, DBA4, RPS17L, RPS17L1 or RPS17L2. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00370 | WB | 100 µl | ||||
| VPA00370KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human RPS17 antibody recognizes RPS17, also known as 40S ribosomal protein S17, DBA4, RPS17L, RPS17L1 and RPS17L2.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. The RPS17 gene encodes a ribosomal protein that is a component of the 40S subunit. RPS17 belongs to the S17E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS17 dispersed through the genome (provided by RefSeq, Jul 2008).
Rabbit anti Human RPS17 antibody detects a band of 19 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. The RPS17 gene encodes a ribosomal protein that is a component of the 40S subunit. RPS17 belongs to the S17E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS17 dispersed through the genome (provided by RefSeq, Jul 2008).
Rabbit anti Human RPS17 antibody detects a band of 19 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RPS17L detects a band of approximately 19 kDa in HepG2 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 77-103 of human RPS17
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P08708 Related reagents
- Entrez Gene
- RPS17 Related reagents
- GO Terms
- GO:0022627 cytosolic small ribosomal subunit
- GO:0003735 structural constituent of ribosome
- GO:0006364 rRNA processing
- GO:0006413 translational initiation
- GO:0006414 translational elongation
- GO:0006415 translational termination
- GO:0019083 viral transcription
- GO:0031018 endocrine pancreas development
- GO:0034101 erythrocyte homeostasis
- GO:0042274 ribosomal small subunit biogenesis
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RPS17 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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