RPL10 antibody

Rabbit anti RPL10

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
RPL10

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VPA00362
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Rabbit anti Human RPL10 antibody recognizes RPL10, also known as 60S ribosomal protein L10, Wilms tumor-related protein, laminin receptor homolog and tumor suppressor QM.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. The RPL10 gene encodes a ribosomal protein that is a component of the 60S subunit. RPL10 belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro., the chicken protein can bind to c-Jun repress c-Jun-mediated transcriptional activation. Alternative splicing results in multiple transcript variants. RPL10 also uses multiple polyA signals, with the 3′-most polyA signal overlapping the deoxyribonuclease I-like 1 gene on the opposite strand. RPL10 is co-transcribed with the small nucleolar RNA gene U70, located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL10 dispersed through the genome (provided by RefSeq, Feb 2012).

Rabbit anti Human RPL10 antibody detects a band of 25 kDa and has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RPL10 detects a band of approximately 25 kDa in NIH/3T3 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 17-45 of human RPL10
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P27635
Entrez Gene
RPL10
GO Terms
GO:0022625 cytosolic large ribosomal subunit
GO:0003735 structural constituent of ribosome
GO:0005783 endoplasmic reticulum
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0019083 viral transcription
GO:0031018 endocrine pancreas development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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