RFI antibody
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Rabbit anti Human RFI
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- RFI
Antibody Quality and Performance Guarantee
| Rabbit anti Human RFI antibodydetects human Ring Finger homologous to Inhibitor of apoptosis protein (hRFI), an anti-apoptotic protein which has been shown to be a novel modulator of NF-kB activation, leading to the upregulation of Bcl2 and BclXL. It is also thought to possess E3 ubiquitin protein ligase activity and has been shown to target caspases 8 and 10 for proteasomal degradation. hRFI is expressed ubiquitously, and has been localized to both the nucleus and the cytoplasm. hRFI is also expressed in oesophageal, gastric and colorectal cancers, suggesting a possible association between hRFI and the development of digestive tract cancers. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence New World primate Expected from Sequence Mouse Expected from Sequence Dog Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human RFI were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein corresponding to amino acids 1-373 of human RFI.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1800 | 1/4000 |
| Western Blotting | |
1/100 | 1/600 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1340 detects a band of approximately 42kDa.
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for RFI antibody
-
Konishi, T. et al. (2006) Overexpression of hRFI inhibits 5-fluorouracil-induced apoptosis in colorectal cancer cells via activation of NF-kappaB and upregulation of BCL-2 and BCL-XL.
Oncogene. 25 (22): 3160-9.
- Synonyms
- hRFI
- RRID
- AB_2253945
- UniProt
- Q969K3
- Entrez Gene
- RNF34
- GO Terms
- GO:0016020 membrane
- GO:0006915 apoptosis
- GO:0008270 zinc ion binding
- GO:0012505 endomembrane system
- GO:0016607 nuclear speck
- GO:0016874 ligase activity
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