RFI antibody

Rabbit anti Human RFI

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RFI

Product Code Applications Pack Size List Price Your Price Qty
AHP1340
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
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Rabbit anti Human RFI antibodydetects human Ring Finger homologous to Inhibitor of apoptosis protein (hRFI), an anti-apoptotic protein which has been shown to be a novel modulator of NF-kB activation, leading to the upregulation of Bcl2 and BclXL. It is also thought to possess E3 ubiquitin protein ligase activity and has been shown to target caspases 8 and 10 for proteasomal degradation.

hRFI is expressed ubiquitously, and has been localized to both the nucleus and the cytoplasm. hRFI is also expressed in oesophageal, gastric and colorectal cancers, suggesting a possible association between hRFI and the development of digestive tract cancers.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
New World primate Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human RFI were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 1-373 of human RFI.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1800 1/4000
Western Blotting 1/100 1/600
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1340 detects a band of approximately 42kDa.

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References for RFI antibody

  1. Konishi, T. et al. (2006) Overexpression of hRFI inhibits 5-fluorouracil-induced apoptosis in colorectal cancer cells via activation of NF-kappaB and upregulation of BCL-2 and BCL-XL.
    Oncogene. 25 (22): 3160-9.

Synonyms
hRFI
RRID
AB_2253945
UniProt
Q969K3
Entrez Gene
RNF34
GO Terms
GO:0016020 membrane
GO:0006915 apoptosis
GO:0008270 zinc ion binding
GO:0012505 endomembrane system
GO:0016607 nuclear speck
GO:0016874 ligase activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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