PTK2B antibody
Rabbit anti PTK2B
PTK2B antibody recognizes protein-tyrosine kinase 2-beta, also known as cell adhesion kinase beta (CAK-beta), focal adhesion kinase 2 (FADK 2), calcium-dependent tyrosine kinase, or calcium-regulated non-receptor proline-rich tyrosine kinase.
PTK2B Antibody with Control Lysate
PTK2B antibody recognizes protein-tyrosine kinase 2-beta, also known as cell adhesion kinase beta (CAK-beta), focal adhesion kinase 2 (FADK 2), calcium-dependent tyrosine kinase, or calcium-regulated non-receptor proline-rich tyrosine kinase.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00237 | WB | 100 µl | ||||
| VPA00237KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human PTK2B antibody recognizes protein-tyrosine kinase 2-beta, also known as CAK-beta, FADK 2, calcium-dependent tyrosine kinase, calcium-regulated non-receptor proline-rich tyrosine kinase, cell adhesion kinase beta, focal adhesion kinase 2, proline-rich tyrosine kinase 2, protein kinase B and related adhesion focal tyrosine kinase.
Encoded by the PTK2B gene, protein-tyrosine kinase 2-beta is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. This protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Protein-tyrosine kinase 2-beta undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. It has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. Protein-tyrosine kinase 2-beta is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for PTK2B (provided by RefSeq, Jul 2008).
Rabbit anti Human PTK2B antibody detects a band of 116 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the PTK2B gene, protein-tyrosine kinase 2-beta is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. This protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Protein-tyrosine kinase 2-beta undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. It has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. Protein-tyrosine kinase 2-beta is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for PTK2B (provided by RefSeq, Jul 2008).
Rabbit anti Human PTK2B antibody detects a band of 116 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PTK2B detects a band of approximately 116 kDa in Raji cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 805-838 amino acids from the C-terminal region of human PTK2B
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Raji lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q14289 Related reagents
- Entrez Gene
- PTK2B Related reagents
- GO Terms
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0004871 signal transducer activity
- GO:0005524 ATP binding
- GO:0007172 signal complex assembly
- GO:0008284 positive regulation of cell proliferation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PTK2B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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