PSMB5 antibody

Rabbit anti PSMB5

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
PSMB5

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VPA00210
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Rabbit anti Human PSMB5 antibody recognizes proteasome subunit beta type-5, also known as PSX large multifunctional protease X, macropain epsilon chain, multicatalytic endopeptidase complex epsilon chain, proteasome catalytic subunit 3, proteasome chain 6, proteasome epsilon chain, proteasome subunit MB1 and proteasome subunit X.

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Encoded by the PSMB5 gene, proteasome subunit beta type-5 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for PSMB5 (provided by RefSeq, Jan 2009).

Rabbit anti Human PSMB5 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PSMB5 detects a band of approximately 22 kDa in MCF7 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide between 235-263 amino acids from the C-terminal region of human PSMB5
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P28074
Entrez Gene
PSMB5
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0006915 apoptosis
GO:0004298 threonine-type endopeptidase activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0016032 viral reproduction
GO:0016071 mRNA metabolic process
GO:0042981 regulation of apoptosis
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0044419 interspecies interaction between organisms
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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