PSMB5 antibody

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Anti PSMB5 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti PSMB5 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti PSMB5 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti PSMB5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PSMB5 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to PSMB5 (molecular weight 22 kDa)

Anti PSMB5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Normalized signal intensity of PSMB5 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMB5 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Anti PSMB5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMB5 (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti PSMB5 antibody (**VPA00210**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, 1705046) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 117 second (chemiluminescent channel) and 30 second

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Rabbit anti PSMB5

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PSMB5

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00210 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human PSMB5 antibody recognizes proteasome subunit beta type-5, also known as PSX large multifunctional protease X, macropain epsilon chain, multicatalytic endopeptidase complex epsilon chain, proteasome catalytic subunit 3, proteasome chain 6, proteasome epsilon chain, proteasome subunit MB1 and proteasome subunit X.

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Encoded by the PSMB5 gene, proteasome subunit beta type-5 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for PSMB5 (provided by RefSeq, Jan 2009).

Rabbit anti Human PSMB5 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PSMB5 detects a band of approximately 22 kDa in MCF7 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 235-263 amino acids from the C-terminal region of human PSMB5
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P28074
Entrez Gene: PSMB5
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0006915 apoptosis; GO:0004298 threonine-type endopeptidase activity; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0006521 regulation of cellular amino acid metabolic process; View More GO Terms; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0016032 viral reproduction; GO:0016071 mRNA metabolic process; GO:0042981 regulation of apoptosis; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0044419 interspecies interaction between organisms; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle