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Prolyl Hydroxylase 1 antibody

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Anti Prolyl Hydroxylase 1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti Prolyl Hydroxylase 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with prolyl hydroxylase 1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 17 second exposure. Arrow points to prolyl hydroxylase 1 (molecular weight 44 kDa)

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Rabbit anti Prolyl Hydroxylase 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Prolyl Hydroxylase 1

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Rabbit anti Human prolyl hydroxylase 1 antibody recognizes prolyl hydroxylase 1, also known as PHD1, EGLN2, HIF-prolyl hydroxylase 1, egl9 homolog 2, hypoxia-inducible factor prolyl hydroxylase 1 and prolyl hydroxylase domain-containing protein 1.

Hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degradation by prolyl hydroxylation. The prolyl hydroxylase 1 gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between the prolyl hydroxylase 1 gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene (provided by RefSeq, Feb 2011).

Rabbit anti Human prolyl hydroxylase 1 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti prolyl hydroxylase 1 detects a band of approximately 44 kDa in HepG2 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 96-123 of human prolyl hydroxylase 1
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
Q96KS0
Entrez Gene
EGLN2
GO Terms
GO:0001558 regulation of cell growth
GO:0001666 response to hypoxia
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0008198 ferrous iron binding
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0019826 oxygen sensor activity
GO:0030520 estrogen receptor signaling pathway
GO:0031418 L-ascorbic acid binding
GO:0045732 positive regulation of protein catabolic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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