Prolyl Hydroxylase 1 antibody

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Rabbit anti Prolyl Hydroxylase 1

Rabbit anti human prolyl hydroxylase 1 antibody recognizes EGL nine homolog 2, also known as PHD1, EGLN2, HIF-prolyl hydroxylase 1, estrogen-induced tag 6, or prolyl hydroxylase domain-containing protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Prolyl Hydroxylase 1 Antibody with Control Lysate

Rabbit anti human prolyl hydroxylase 1 antibody recognizes EGL nine homolog 2, also known as PHD1, EGLN2, HIF-prolyl hydroxylase 1, estrogen-induced tag 6, hypoxia-inducible factor prolyl hydroxylase 1 or prolyl hydroxylase domain-containing protein 1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00264 WB 100 µl
VPA00264KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human prolyl hydroxylase 1 antibody recognizes prolyl hydroxylase 1, also known as PHD1, EGLN2, HIF-prolyl hydroxylase 1, egl9 homolog 2, hypoxia-inducible factor prolyl hydroxylase 1 and prolyl hydroxylase domain-containing protein 1.

Hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degradation by prolyl hydroxylation. The prolyl hydroxylase 1 gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between the prolyl hydroxylase 1 gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene (provided by RefSeq, Feb 2011).

Rabbit anti Human prolyl hydroxylase 1 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti prolyl hydroxylase 1 detects a band of approximately 44 kDa in HepG2 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 96-123 of human prolyl hydroxylase 1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q96KS0 Related reagents
Entrez Gene
EGLN2 Related reagents
GO Terms
GO:0001558 regulation of cell growth
GO:0001666 response to hypoxia
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0008198 ferrous iron binding
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0019826 oxygen sensor activity
GO:0030520 estrogen receptor signaling pathway
GO:0031418 L-ascorbic acid binding
GO:0045732 positive regulation of protein catabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Prolyl Hydroxylase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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