Prolyl Hydroxylase 1 antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti Prolyl Hydroxylase 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with prolyl hydroxylase 1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 17 second exposure. Arrow points to prolyl hydroxylase 1 (molecular weight 44 kDa)

Filter by Application:

Rabbit anti Prolyl Hydroxylase 1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Prolyl Hydroxylase 1

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00264 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Rabbit anti Human prolyl hydroxylase 1 antibody recognizes prolyl hydroxylase 1, also known as PHD1, EGLN2, HIF-prolyl hydroxylase 1, egl9 homolog 2, hypoxia-inducible factor prolyl hydroxylase 1 and prolyl hydroxylase domain-containing protein 1.

Hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degradation by prolyl hydroxylation. The prolyl hydroxylase 1 gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between the prolyl hydroxylase 1 gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene (provided by RefSeq, Feb 2011).

Rabbit anti Human prolyl hydroxylase 1 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti prolyl hydroxylase 1 detects a band of approximately 44 kDa in HepG2 cell lysate.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH-conjugated synthetic peptide corresponding to aa 96-123 of human prolyl hydroxylase 1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q96KS0
Entrez Gene: EGLN2
GO Terms: GO:0001558 regulation of cell growth; GO:0001666 response to hypoxia; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0055114 oxidation-reduction process; GO:0045454 cell redox homeostasis; GO:0008198 ferrous iron binding; GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; View More GO Terms; GO:0019826 oxygen sensor activity; GO:0030520 estrogen receptor signaling pathway; GO:0031418 L-ascorbic acid binding; GO:0045732 positive regulation of protein catabolic process