PPP2R2A antibody

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Rabbit anti PPP2R2A

Rabbit anti human PPP2R2A antibody recognizes serine/threonine-protein phosphatase 2A regulatory subunit B alpha isoform, also known as protein phosphatase 2 (formerly 2A), or serine/threonine-protein phosphatase 2A. This antibody has been extensively validated for western blotting.

PPP2R2A Antibody with Control Lysate

Rabbit anti human PPP2R2A antibody recognizes serine/threonine-protein phosphatase 2A regulatory subunit B alpha isoform, also known as protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52), alpha isoform or serine/threonine-protein phosphatase 2A.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00328 WB 100 µl
VPA00328KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human PPP2R2A antibody recognizes PPP2R2A, also known as protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52) alpha isoform, 55 kDa regulatory subunit B, alpha isoform and serine/threonine-protein phosphatase 2A.

The product of the PPP2R2A gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, which associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. The PPP2R2A gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described (provided by RefSeq, Apr 2010).

Rabbit anti Human PPP2R2A antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PPP2R2A detects a band of approximately 52 kDa in HeLa cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 126-160 of human PPP2R2A
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P63151 Related reagents
Entrez Gene
PPP2R2A Related reagents
GO Terms
GO:0000159 protein phosphatase type 2A complex
GO:0005515 protein binding
GO:0004722 protein serine/threonine phosphatase activity
GO:0007165 signal transduction
GO:0006470 protein dephosphorylation
GO:0008601 protein phosphatase type 2A regulator activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PPP2R2A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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