PPAR Gamma antibody
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Rabbit antii Human PPAR gamma antibody detects human peroxisome proliferator-activated receptor γ (PPARG), a member of the nuclear hormone receptor subfamily of transcription factors. Upon activation, PPARG forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. PPARG is principally involved in the cellular assimilation of lipids, with key roles in adipocyte differentiation and adipogenesis.
Two alternatively spliced isoforms of PPARG exist, termed PPARG1 and PPARG2, which are identical except for an additional 30 amino acids at the N-terminus of PPARG2. This results in a predominant adipocyte localisation for PPARG2, whilst PPARG1 is ubiquitously expressed.
PPARG has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer.
Two alternatively spliced isoforms of PPARG exist, termed PPARG1 and PPARG2, which are identical except for an additional 30 amino acids at the N-terminus of PPARG2. This results in a predominant adipocyte localisation for PPARG2, whilst PPARG1 is ubiquitously expressed.
PPARG has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Dog Expected from Sequence Hamster Expected from Sequence Rabbit Expected from Sequence Mink Expected from Sequence Rat Expected from Sequence Guinea Pig Expected from Sequence Macaque Expected from Sequence Duck Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to PPAR gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to amino acids 255 - 268 of human PPAR gamma isoform 1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P37231 Related reagents
- Entrez Gene
- PPARG Related reagents
- Regulatory
- For research purposes only
Applications of PPAR Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/8000 | 1/32000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Product Specific References
References for PPAR Gamma antibody
-
Wu, H. et al. (2017) Decreased PPAR-γ expression after internal carotid artery stenting is associated with vascular lesions induced by smooth muscle cell proliferation and systemic inflammation in a minipig model.
Int J Clin Exp Pathol 2017;10(7): 7375-83.
Further Reading
-
Lazar, M.A. (2005) PPAR gamma, 10 years later.
Biochimie. 87 (1): 9-13. -
Lehrke, M. & Lazar, M.A. (2005) The many faces of PPARgamma.
Cell. 123 (6): 993-9.
