PPAR Gamma antibody

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Rabbit anti PPAR Gamma

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1269 E 50 µg
Rabbit antii Human PPAR gamma antibody detects human peroxisome proliferator-activated receptor γ (PPARG), a member of the nuclear hormone receptor subfamily of transcription factors. Upon activation, PPARG forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. PPARG is principally involved in the cellular assimilation of lipids, with key roles in adipocyte differentiation and adipogenesis.

Two alternatively spliced isoforms of PPARG exist, termed PPARG1 and PPARG2, which are identical except for an additional 30 amino acids at the N-terminus of PPARG2. This results in a predominant adipocyte localisation for PPARG2, whilst PPARG1 is ubiquitously expressed.

PPARG has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
Hamster Expected from Sequence
Rabbit Expected from Sequence
Mink Expected from Sequence
Rat Expected from Sequence
Guinea Pig Expected from Sequence
Macaque Expected from Sequence
Duck Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to PPAR gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 255 - 268 of human PPAR gamma isoform 1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P37231 Related reagents
Entrez Gene
PPARG Related reagents
Regulatory
For research purposes only

Applications of PPAR Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000 1/32000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for PPAR Gamma antibody

  1. Wu, H. et al. (2017) Decreased PPAR-γ expression after internal carotid artery stenting is associated with vascular lesions induced by smooth muscle cell proliferation and systemic inflammation in a minipig model.
    Int J Clin Exp Pathol 2017;10(7): 7375-83.

Further Reading

  1. Lazar, M.A. (2005) PPAR gamma, 10 years later.
    Biochimie. 87 (1): 9-13.
  2. Lehrke, M. & Lazar, M.A. (2005) The many faces of PPARgamma.
    Cell. 123 (6): 993-9.