Rabbit anti POLRMT antibody recognizes DNA-directed RNA polymerase, mitochondrial, also known as MtRPOL, MTRNAP, and APOLMT.
POLRMT is a mitochondrial RNA polymerase absolutely required for expression of mitochondrial genes, and required for replication of the mitochondrial genome (Kühl et al. 2016). Evidence does not support the existence of a nuclear isoform of POLRMT, suggesting that its role is exclusively mitochondrial (Kühl et al. 2014).
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to POLRMT was raised by repeated immunization of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3)
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
DNA-directed RNA polymerase activity
transcription initiation from mitochondrial promoter
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Polrmt antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available