PCNA antibody
Rabit anti Human PCNA antibody (AHP1419) used for the identification of proliferating cells in the embryos of the Nile crocodile (Crocodylus niloticus), Bearded dragon (Pogona vitticeps) and Corn snake (Pantherophis guttatus) by immunofluorescence.
Image caption:
Development of epidermal scales during reptilian embryogenesis.
(A) Hematoxylin and eosin (H&E) staining of skin sections from different body regions (indicated with red arrows on the top insets with lateral views of corresponding embryos) of C. niloticus (crocodile; top row), P.vitticeps (lizard; two middle rows), and P. guttatus (snake; bottom row) embryos at various developmental stages [indicated as embryonic days (E) after oviposition]. White arrowheads indicate the anatomical placode. Scale bars, 100 μm. (B) Anatomical placodes in C. niloticus (left panels), P.vitticeps (middle panels), and P. guttatus (right panels) embryos. For each species, the whole-embryo WMISH with Sonic hedgehog (Shh) is shown (left panel) as well as, from top to bottom, high magnification of H&E-stained placode sections (white arrowheads indicate placode columnar cells), immunohistochemistry with PCNA (proliferation marker; epidermal-dermal junction indicated by dashed white lines), and parasagittal cryosections of placodes after Shh or β-catenin (Ctnnb1) WMISH. Bmp4 is also shown for lizard. Red double-headed arrows indicate the body region processed for sectioning.
From: Di-Poi, N & Milinkovitch, M.C. (2016)
The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2: 1600708.
doi: 10.1126/sciadv.1600708
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human PCNA antibody (AHP1419) used to evaluate PCNA expression in mouse tumor cells by immunofluorescence.
Image caption:
Inhibition of tumor growth by melittin–dKLA in a mouse model of melanoma. (D) Expression of PCNA in tumor tissues was determined by immunofluorescence staining assay. Confocal images of a tumor tissue slice stained for PCNA (green) and nucleus (DAPI; blue). (E) The graph is presented as intensity relative to PBS. All data are presented as means ± SD (n = 5); * p <0.05, *** p <0.0001 versus the PBS group, MK: melittin–dKLA.
From: Han, I.-H.; Jeong, C.; Yang, J.; Park, S.-H.; Hwang, D.-S.; Bae, H.
Therapeutic Effect of Melittin–dKLA Targeting Tumor-Associated Macrophages in Melanoma.
Int J Mol Sci. 2022, 23, 3094.
doi: 10.3390/ijms23063094
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB P IF ResetRabbit anti PCNA
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- PCNA
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| Rabbit anti Human PCNA antibody recognizes Human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36 kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair (Burkovics et al. 2009). PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohistochemistry on paraffin sections using rabbit anti human PCNA antibody (Gunin et al. 2014). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chicken Fish Expected from Sequence Rat Amphibia Expected from Sequence Bovine Monkey Mouse Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human PCNA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding an internal region of human PCNA protein.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/2000 | 1/8000 |
| Immunohistology - Paraffin 1 | |
2.5ug/ml | |
| Western Blotting | |
1/500 | 1/2000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Breast
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References for PCNA antibody
-
Gunin, A.G. et al. (2010) Age-Related Changes in Proliferation, the Numbers of Mast Cells, Eosinophils, and cd45-Positive Cells in Human Dermis
J Gerontol A Biol Sci Med Sci. 66: 385-92. -
Kim, J.Y. et al. (2012) Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats.
Angle Orthod. 82: 107-14. -
Petrov. V.V. et al. (2013) Age-related changes in mast cells and eosinophils of human dermis
Ontogenez. 44: 179-85. -
Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
Exp Gerontol. 55C: 143-51. -
Di-Poi, N. & Milinkovitch, M. C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes
Science Advances. 2 (6): e1600708. -
Gunin, A.G. et al. (2020) [Arylhydrocarbon receptor nuclear translocator (ARNT) in human skin during aging.]
Adv Gerontol. 33 (2): 313-8. -
Han, I. et al. (2022) Therapeutic Effect of Melittin–dKLA Targeting Tumor-Associated Macrophages in Melanoma
Int J Med Sci. 23 (6): 3094.
- Synonyms
- Proliferating Cell Nuclear Antigen
- RRID
- AB_2252059
- UniProt
- P12004
- Entrez Gene
- PCNA
- GO Terms
- GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity
- GO:0000722 telomere maintenance via recombination
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0005663 DNA replication factor C complex
- GO:0043626 PCNA complex
- GO:0006271 DNA strand elongation involved in DNA replication
- View More GO Terms
- GO:0006275 regulation of DNA replication
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006297 nucleotide-excision repair, DNA gap filling
- GO:0006298 mismatch repair
- GO:0008283 cell proliferation
- GO:0015630 microtubule cytoskeleton
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0019985 translesion synthesis
- GO:0030337 DNA polymerase processivity factor activity
- GO:0032077 positive regulation of deoxyribonuclease activity
- GO:0032139 dinucleotide insertion or deletion binding
- GO:0032201 telomere maintenance via semi-conservative replication
- GO:0032405 MutLalpha complex binding
- GO:0043596 nuclear replication fork
- GO:0070557 PCNA-p21 complex
View more products with PCNA specificity
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