PCNA antibody

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Anti PCNA Antibody gallery image 1 — Western blot analysis of PCNA expression in Jurkat (lane 1) and HEK293 (lane 2) whole cell lysates using Rabbit anti Human PCNA polyclonal antibody (AHP1419) followed by goat anti Rabbit IgG and visualized by immunofluorescence

Anti PCNA Antibody gallery image 2 — Staining of formalin fixed,paraffin embedded human breast tissue section with Rabbit anti Human PCNA antibody (**AHP1419**).

Anti PCNA Antibody gallery image 3 — **Published customer image:**
Rabit anti Human PCNA antibody (**AHP1419**) used for the identification of proliferating cells in the embryos of the Nile crocodile (*Crocodylus niloticus*), Bearded dragon (*Pogona vitticeps*) and Corn snake (*Pantherophis guttatus*) by immunofluorescence.
**Image caption:**
Development of epidermal scales during reptilian embryogenesis.
(A) Hematoxylin and eosin (H&E) staining of skin sections from different body regions (indicated with red arrows on the top insets with lateral views of corresponding embryos) of *C. niloticus* (crocodile; top row), *P.vitticeps* (lizard; two middle rows), and P. guttatus (snake; bottom row) embryos at various developmental stages [indicated as embryonic days (E) after oviposition]. White arrowheads indicate the anatomical placode. Scale bars, 100 μm. (B) Anatomical placodes in *C. niloticus* (left panels), *P.vitticeps* (middle panels), and P. guttatus (right panels) embryos. For each species, the whole-embryo WMISH with Sonic hedgehog (Shh) is shown (left panel) as well as, from top to bottom, high magnification of H&E-stained placode sections (white arrowheads indicate placode columnar cells), immunohistochemistry with PCNA (proliferation marker; epidermal-dermal junction indicated by dashed white lines), and parasagittal cryosections of placodes after Shh or β-catenin (Ctnnb1) WMISH. Bmp4 is also shown for lizard. Red double-headed arrows indicate the body region processed for sectioning.

From: Di-Poi, N & Milinkovitch, M.C. (2016)
The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2: 1600708.
doi: 10.1126/sciadv.1600708
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti PCNA

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1419 Datasheet	E P* WB SDS	50 µg	Log in		Get Quote

Rabbit anti Human PCNA antibody recognizes Human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36 kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair (Burkovics et al. 2009).

PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohistochemistry on paraffin sections using rabbit anti human PCNA antibody (Gunin et al. 2014).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Chicken
Fish	Expected from Sequence
Rat
Amphibia	Expected from Sequence
Bovine
Monkey
Mouse
Dog

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human PCNA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN₃)

Immunogen

Synthetic peptide corresponding an internal region of human PCNA protein.

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P12004
Entrez Gene: PCNA
GO Terms: GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity; GO:0000722 telomere maintenance via recombination; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0005663 DNA replication factor C complex; GO:0043626 PCNA complex; GO:0006271 DNA strand elongation involved in DNA replication; GO:0006275 regulation of DNA replication; GO:0006283 transcription-coupled nucleotide-excision repair; GO:0006297 nucleotide-excision repair, DNA gap filling; GO:0006298 mismatch repair; GO:0008283 cell proliferation; GO:0015630 microtubule cytoskeleton; GO:0048015 phosphatidylinositol-mediated signaling; GO:0019985 translesion synthesis; GO:0030337 DNA polymerase processivity factor activity; GO:0032077 positive regulation of deoxyribonuclease activity; GO:0032139 dinucleotide insertion or deletion binding; GO:0032201 telomere maintenance via semi-conservative replication; GO:0032405 MutLalpha complex binding; GO:0043596 nuclear replication fork; GO:0070557 PCNA-p21 complex
Regulatory: For research purposes only

Applications of PCNA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/2000	1/8000
Immunohistology - Paraffin ¹		2.5ug/ml
Western Blotting	1/500	1/2000

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Breast

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in	Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in	Get Quote
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for PCNA antibody

Gunin, A.G. et al. (2010) Age-Related Changes in Proliferation, the Numbers of Mast Cells, Eosinophils, and cd45-Positive Cells in Human Dermis
J Gerontol A Biol Sci Med Sci. 66: 385-92.
Kim, J.Y. et al. (2012) Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats.
Angle Orthod. 82: 107-14.
Petrov. V.V. et al. (2013) Age-related changes in mast cells and eosinophils of human dermis
Ontogenez. 44: 179-85.
Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
Exp Gerontol. 55C: 143-51.
Di-Poi, N. & Milinkovitch, M. C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes
Science Advances. 2 (6): e1600708.

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