PCNA antibody



PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohustochemistry on paraffin sections using rabbit anti human PCNA antibody (Gunin et al. 2014).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chicken Fish Expected from Sequence Rat Amphibia Expected from Sequence Bovine Monkey Mouse Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human PCNA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding an internal region of human PCNA protein.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P12004
- Entrez Gene
- PCNA
- GO Terms
- GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity
- GO:0000722 telomere maintenance via recombination
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0005663 DNA replication factor C complex
- GO:0043626 PCNA complex
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006275 regulation of DNA replication
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006297 nucleotide-excision repair, DNA gap filling
- GO:0006298 mismatch repair
- GO:0008283 cell proliferation
- GO:0015630 microtubule cytoskeleton
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0019985 translesion synthesis
- GO:0030337 DNA polymerase processivity factor activity
- GO:0032077 positive regulation of deoxyribonuclease activity
- GO:0032139 dinucleotide insertion or deletion binding
- GO:0032201 telomere maintenance via semi-conservative replication
- GO:0032405 MutLalpha complex binding
- GO:0043596 nuclear replication fork
- GO:0070557 PCNA-p21 complex
- Regulatory
- For research purposes only
Applications of PCNA antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/2000 | 1/8000 | |
Immunohistology - Paraffin 1 | 2.5ug/ml | ||
Western Blotting | 1/500 | 1/2000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Breast
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for PCNA antibody
-
Gunin, A.G. et al. (2010) Age-Related Changes in Proliferation, the Numbers of Mast Cells, Eosinophils, and cd45-Positive Cells in Human Dermis
J Gerontol A Biol Sci Med Sci. 66: 385-92. -
Kim, J.Y. et al. (2012) Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats.
Angle Orthod. 82: 107-14. -
Petrov. V.V. et al. (2013) Age-related changes in mast cells and eosinophils of human dermis
Ontogenez. 44: 179-85. -
Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
Exp Gerontol. 55C: 143-51. -
Di-Poi, N. & Milinkovitch, M. C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes
Science Advances. 2 (6): e1600708.
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