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PCNA antibody

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Anti PCNA Antibody thumbnail image 1 Anti PCNA Antibody thumbnail image 2 Anti PCNA Antibody thumbnail image 3
Anti PCNA Antibody gallery image 1
Staining of formalin fixed,paraffin embedded human breast with Rabbit anti Human PCNA (AHP1419)
Anti PCNA Antibody gallery image 2
Western blot analysis of PCNA expression in Jurkat (lane 1) and HEK293 (lane 2) whole cell lysates using Rabbit anti Human PCNA polyclonal antibody (AHP1419) followed by goat anti Rabbit IgG and visualized by immunofluorescence
Anti PCNA Antibody gallery image 3
Published customer image:
Rabit anti Human PCNA antibody (AHP1419) used for the identification of proliferating cells in the embryos of the Nile crocodile (Crocodylus niloticus), Bearded dragon (Pogona vitticeps) and Corn snake (Pantherophis guttatus) by immunofluorescence.
Image caption:
Development of epidermal scales during reptilian embryogenesis.
(A) Hematoxylin and eosin (H&E) staining of skin sections from different body regions (indicated with red arrows on the top insets with lateral views of corresponding embryos) of C. niloticus (crocodile; top row), P.vitticeps (lizard; two middle rows), and P. guttatus (snake; bottom row) embryos at various developmental stages [indicated as embryonic days (E) after oviposition]. White arrowheads indicate the anatomical placode. Scale bars, 100 μm. (B) Anatomical placodes in C. niloticus (left panels), P.vitticeps (middle panels), and P. guttatus (right panels) embryos. For each species, the whole-embryo WMISH with Sonic hedgehog (Shh) is shown (left panel) as well as, from top to bottom, high magnification of H&E-stained placode sections (white arrowheads indicate placode columnar cells), immunohistochemistry with PCNA (proliferation marker; epidermal-dermal junction indicated by dashed white lines), and parasagittal cryosections of placodes after Shh or β-catenin (Ctnnb1) WMISH. Bmp4 is also shown for lizard. Red double-headed arrows indicate the body region processed for sectioning.

From: Di-Poi, N & Milinkovitch, M.C. (2016)
The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2: 1600708.

Rabbit anti PCNA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1419
Datasheet
E P* WB
SDS
50 µg loader

Rabbit anti Human PCNA antibody recognizes Human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36 kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair (Burkovics et al. 2009).

PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohustochemistry on paraffin sections using rabbit anti human PCNA antibody (Gunin et al. 2014).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Fish Expected from Sequence
Rat
Amphibia Expected from Sequence
Bovine
Monkey
Mouse
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human PCNA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding an internal region of human PCNA protein.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P12004
Entrez Gene
PCNA
GO Terms
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity
GO:0000722 telomere maintenance via recombination
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005663 DNA replication factor C complex
GO:0043626 PCNA complex
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006275 regulation of DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0006298 mismatch repair
GO:0008283 cell proliferation
GO:0015630 microtubule cytoskeleton
GO:0048015 phosphatidylinositol-mediated signaling
GO:0019985 translesion synthesis
GO:0030337 DNA polymerase processivity factor activity
GO:0032077 positive regulation of deoxyribonuclease activity
GO:0032139 dinucleotide insertion or deletion binding
GO:0032201 telomere maintenance via semi-conservative replication
GO:0032405 MutLalpha complex binding
GO:0043596 nuclear replication fork
GO:0070557 PCNA-p21 complex
Regulatory
For research purposes only

Applications of PCNA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/8000
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 1/500 1/2000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Breast
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for PCNA antibody

  1. Gunin, A.G. et al. (2010) Age-Related Changes in Proliferation, the Numbers of Mast Cells, Eosinophils, and cd45-Positive Cells in Human Dermis
    J Gerontol A Biol Sci Med Sci. 66: 385-92.
  2. Kim, J.Y. et al. (2012) Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats.
    Angle Orthod. 82: 107-14.
  3. Petrov. V.V. et al. (2013) Age-related changes in mast cells and eosinophils of human dermis
    Ontogenez. 44: 179-85.
  4. Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
    Exp Gerontol. 55C: 143-51.
  5. Di-Poi, N. & Milinkovitch, M. C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes
    Science Advances. 2 (6): e1600708.

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