p63 antibody

p63, vital for the development of the prostate and selectively expressed by normal prostate basal cells, has been shown to be a promising complementary basal cell specific marker to High Molecular Weight-Cytokeratin (HMW-CK), for the differential diagnosis of benign prostatic lesions and prostatic carcinoma.
Rabbit anti p63 antibody is reported as suitable for use in immunocytochemistry.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human p63 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin - Immunogen
- Synthetic peptide C-NFDMDARRNKQQRIKEEGE corresponding to amino acids 662-680 within the C-terminal region of p63.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9H3D4
- O88898
- Entrez Gene
- Trp63
- TP63
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0005515 protein binding
- GO:0001501 skeletal system development
- GO:0001736 establishment of planar polarity
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0002064 epithelial cell development
- GO:0002347 response to tumor cell
- GO:0003682 chromatin binding
- GO:0003684 damaged DNA binding
- GO:0005634 nucleus
- GO:0006916 anti-apoptosis
- GO:0007219 Notch signaling pathway
- GO:0007499 ectoderm and mesoderm interaction
- GO:0008270 zinc ion binding
- GO:0009954 proximal/distal pattern formation
- GO:0010259 multicellular organismal aging
- GO:0030216 keratinocyte differentiation
- GO:0030308 negative regulation of cell growth
- GO:0030326 embryonic limb morphogenesis
- GO:0030859 polarized epithelial cell differentiation
- GO:0031069 hair follicle morphogenesis
- GO:0035121 tail morphogenesis
- GO:0042475 odontogenesis of dentine-containing tooth
- GO:0042771 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
- GO:0043565 sequence-specific DNA binding
- GO:0043589 skin morphogenesis
- GO:0043616 keratinocyte proliferation
- GO:0045617 negative regulation of keratinocyte differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048485 sympathetic nervous system development
- GO:0048745 smooth muscle tissue development
- GO:0048807 female genitalia morphogenesis
- GO:0051402 neuron apoptosis
- GO:0060157 urinary bladder development
- GO:0060197 cloacal septation
- GO:0060513 prostatic bud formation
- GO:0060529 squamous basal epithelial stem cell differentiation involved in prostate gland acinus development
- Regulatory
- For research purposes only
Applications of p63 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/100000 | ||
Western Blotting | 1/1000 |
- Western Blotting
- Rabbit anti Human p63 antibody detects a band of approximately 75kDa in mouse lung and human tumour cell line K562 (protein degredation is as expected).
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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