Rabbit anti p63 antibody recognizes an epitope within the C-terminal (CT) region of tumour protein p63, otherwise known as tumour protein p73-like (p73L), a widely expressed nuclear protein and homologue of tumour suppressor p53, which acts as a sequence-specific DNA binding transcriptional activator/repressor.
p63, vital for the development of the prostate and selectively expressed by normal prostate basal cells, has been shown to be a promising complementary basal cell specific marker to High Molecular Weight-Cytokeratin (HMW-CK), for the differential diagnosis of benign prostatic lesions and prostatic carcinoma.
Rabbit anti p63 antibody is reported as suitable for use in immunocytochemistry.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human p63 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
- Synthetic peptide C-NFDMDARRNKQQRIKEEGE corresponding to amino acids 662-680 within the C-terminal region of p63.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
establishment of planar polarity
positive regulation of mesenchymal cell proliferation
epithelial cell development
response to tumor cell
damaged DNA binding
Notch signaling pathway
ectoderm and mesoderm interaction
zinc ion binding
proximal/distal pattern formation
multicellular organismal aging
negative regulation of cell growth
embryonic limb morphogenesis
polarized epithelial cell differentiation
hair follicle morphogenesis
odontogenesis of dentine-containing tooth
DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
sequence-specific DNA binding
negative regulation of keratinocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
sympathetic nervous system development
smooth muscle tissue development
female genitalia morphogenesis
urinary bladder development
prostatic bud formation
squamous basal epithelial stem cell differentiation involved in prostate gland acinus development
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of p63 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti Human p63 antibody detects a band of approximately 75kDa in mouse lung and human tumour cell line K562 (protein degredation is as expected).
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Secondary Antibodies Available
Useful Reagents Available
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