p63 antibody
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Rabbit anti p63 antibody recognizes an epitope within the C-terminal (CT) region of tumour protein p63, otherwise known as tumour protein p73-like (p73L), a widely expressed nuclear protein and homologue of tumour suppressor p53, which acts as a sequence-specific DNA binding transcriptional activator/repressor.
p63, vital for the development of the prostate and selectively expressed by normal prostate basal cells, has been shown to be a promising complementary basal cell specific marker to High Molecular Weight-Cytokeratin (HMW-CK), for the differential diagnosis of benign prostatic lesions and prostatic carcinoma.
Rabbit anti p63 antibody is reported as suitable for use in immunocytochemistry.
p63, vital for the development of the prostate and selectively expressed by normal prostate basal cells, has been shown to be a promising complementary basal cell specific marker to High Molecular Weight-Cytokeratin (HMW-CK), for the differential diagnosis of benign prostatic lesions and prostatic carcinoma.
Rabbit anti p63 antibody is reported as suitable for use in immunocytochemistry.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human p63 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin - Immunogen
- Synthetic peptide C-NFDMDARRNKQQRIKEEGE corresponding to amino acids 662-680 within the C-terminal region of p63.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- Q9H3D4
- O88898
- Entrez Gene
- Trp63
- TP63
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0005515 protein binding
- GO:0001501 skeletal system development
- GO:0001736 establishment of planar polarity
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0002064 epithelial cell development
- GO:0002347 response to tumor cell
- GO:0003682 chromatin binding
- GO:0003684 damaged DNA binding
- GO:0005634 nucleus
- GO:0006916 anti-apoptosis
- GO:0007219 Notch signaling pathway
- GO:0007499 ectoderm and mesoderm interaction
- GO:0008270 zinc ion binding
- GO:0009954 proximal/distal pattern formation
- GO:0010259 multicellular organismal aging
- GO:0030216 keratinocyte differentiation
- GO:0030308 negative regulation of cell growth
- GO:0030326 embryonic limb morphogenesis
- GO:0030859 polarized epithelial cell differentiation
- GO:0031069 hair follicle morphogenesis
- GO:0035121 tail morphogenesis
- GO:0042475 odontogenesis of dentine-containing tooth
- GO:0042771 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
- GO:0043565 sequence-specific DNA binding
- GO:0043589 skin morphogenesis
- GO:0043616 keratinocyte proliferation
- GO:0045617 negative regulation of keratinocyte differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048485 sympathetic nervous system development
- GO:0048745 smooth muscle tissue development
- GO:0048807 female genitalia morphogenesis
- GO:0051402 neuron apoptosis
- GO:0060157 urinary bladder development
- GO:0060197 cloacal septation
- GO:0060513 prostatic bud formation
- GO:0060529 squamous basal epithelial stem cell differentiation involved in prostate gland acinus development
- Regulatory
- For research purposes only
Applications of p63 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100000 | ||
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti Human p63 antibody detects a band of approximately 75kDa in mouse lung and human tumour cell line K562 (protein degredation is as expected).
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
