p63 antibody

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Rabbit anti p63 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1815 E WB 0.1 mg
Rabbit anti p63 antibody recognizes an epitope within the C-terminal (CT) region of tumour protein p63, otherwise known as tumour protein p73-like (p73L), a widely expressed nuclear protein and homologue of tumour suppressor p53, which acts as a sequence-specific DNA binding transcriptional activator/repressor.

p63, vital for the development of the prostate and selectively expressed by normal prostate basal cells, has been shown to be a promising complementary basal cell specific marker to High Molecular Weight-Cytokeratin (HMW-CK), for the differential diagnosis of benign prostatic lesions and prostatic carcinoma.

Rabbit anti p63 antibody is reported as suitable for use in immunocytochemistry.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human p63 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Synthetic peptide C-NFDMDARRNKQQRIKEEGE corresponding to amino acids 662-680 within the C-terminal region of p63.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9H3D4 Related reagents
O88898 Related reagents
Entrez Gene
Trp63 Related reagents
TP63 Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005515 protein binding
GO:0001501 skeletal system development
GO:0001736 establishment of planar polarity
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0002064 epithelial cell development
GO:0002347 response to tumor cell
GO:0003682 chromatin binding
GO:0003684 damaged DNA binding
GO:0005634 nucleus
GO:0006916 anti-apoptosis
GO:0007219 Notch signaling pathway
GO:0007499 ectoderm and mesoderm interaction
GO:0008270 zinc ion binding
GO:0009954 proximal/distal pattern formation
GO:0010259 multicellular organismal aging
GO:0030216 keratinocyte differentiation
GO:0030308 negative regulation of cell growth
GO:0030326 embryonic limb morphogenesis
GO:0030859 polarized epithelial cell differentiation
GO:0031069 hair follicle morphogenesis
GO:0035121 tail morphogenesis
GO:0042475 odontogenesis of dentine-containing tooth
GO:0042771 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
GO:0043565 sequence-specific DNA binding
GO:0043589 skin morphogenesis
GO:0043616 keratinocyte proliferation
GO:0045617 negative regulation of keratinocyte differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048485 sympathetic nervous system development
GO:0048745 smooth muscle tissue development
GO:0048807 female genitalia morphogenesis
GO:0051402 neuron apoptosis
GO:0060157 urinary bladder development
GO:0060197 cloacal septation
GO:0060513 prostatic bud formation
GO:0060529 squamous basal epithelial stem cell differentiation involved in prostate gland acinus development
Regulatory
For research purposes only

Applications of p63 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100000
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti Human p63 antibody detects a band of approximately 75kDa in mouse lung and human tumour cell line K562 (protein degredation is as expected).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*