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p62 (pThr269/pSer272) antibody

Rabbit anti p62 (pThr269/pSer272)

Product Type
Polyclonal Antibody
Polyclonal IgG
p62 (pThr269/pSer272)

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
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Rabbit anti p62 (pThr269/pSer272) antibody recognizes ubiquitin-binding protein p62, also known as sequestosome-1 and EBI3-associated protein of 60 kDa (EBIAP), when phosphorylated at threonine 269 and serine 272. p62 is important in regulating critical cellular functions, including bone homeostasis, obesity, and cancer via its interactions with various signaling intermediaries. The SQSTM1 gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. p62 functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals.

p62 plays an important role in bone remodeling, regulating this process through its role in a chemical signaling pathway that promotes the formation of osteoclasts. p62 may be involved autophagy (Zhang et al. 2016), the self-destruction of cells (apoptosis), and the body's immune responses and inflammatory reactions.

p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease (Kurosawa et al. 2016). p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages(Wang et al. 2014).

p62 is overexpressed in many human cancers and is induced during cell transformation. cdk1 phosphorylates p62 in vitro and in vivo at T269 and S272, which is necessary for the maintenance of appropriate cyclin B1 levels and the levels of cdk1 activity necessary to allow cells to properly enter and exit mitosis (Linares et al. 2011). The lack of cdk1-mediated phosphorylation of p62 leads to a faster exit from mitosis, translating into enhanced cell proliferation and tumorigenesis in response to Ras-induced transformation.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to p62 (pThr269/pSer272) was raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr269/Ser272 region of human p62
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti Human p62 (pThr269/pSer272) detects a band of approximately 62 kDa in Jurkat cell lysate

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Entrez Gene
GO Terms
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0008104 protein localization
GO:0005080 protein kinase C binding
GO:0005654 nucleoplasm
GO:0005770 late endosome
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006916 anti-apoptosis
GO:0030971 receptor tyrosine kinase binding
GO:0008270 zinc ion binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0016197 endosome transport
GO:0016236 macroautophagy
GO:0030154 cell differentiation
GO:0042169 SH2 domain binding
GO:0043122 regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043130 ubiquitin binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048011 nerve growth factor receptor signaling pathway


162353 170418

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