Orexin A antibody
Sheep anti Human orexin A antibody recognizes the human orexin A subunit of orexin, also known as hypocretin. Orexin is cleaved into Orexin A, also known as hypocretin-1, a 32 amino acid excitatory neuropeptide linked to alertness and wakefulness in humans. Orexin A binds to both orexin receptors 1 and 2 with similar affinity whilst orexin B corresponding to the C terminal region of the holopeptide binds only to the orexin receptor 2.
Mutations in the orexin gene can lead to the development of Narcolepsy 1 (NRCLP1), a condition characterized by sleep fragmentation and daytime drowziness, abnormal REM sleep and sleep paralysis along with other sleep related symptoms (Peyron et al. 2000).
Sheep anti Human orexin A antibody is a useful reagent for immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Mutations in the orexin gene can lead to the development of Narcolepsy 1 (NRCLP1), a condition characterized by sleep fragmentation and daytime drowziness, abnormal REM sleep and sleep paralysis along with other sleep related symptoms (Peyron et al. 2000).
Sheep anti Human orexin A antibody is a useful reagent for immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Expected from Sequence Rat Expected from Sequence Rabbit Expected from Sequence Pig Expected from Sequence Mouse Expected from Sequence Horse Expected from Sequence Dog Expected from Sequence Chimpanzee Expected from Sequence Bovine Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human orexin A conjugated to KLH.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O43612
- Entrez Gene
- HCRT
- GO Terms
- GO:0005576 extracellular region
- GO:0005791 rough endoplasmic reticulum
- GO:0007218 neuropeptide signaling pathway
- GO:0008021 synaptic vesicle
- GO:0030054 cell junction
- Regulatory
- For research purposes only
Applications of Orexin A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/20 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Hypothalamus
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
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| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
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