Orexin A antibody

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Sheep anti Human Orexin A

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
7049-5020
Datasheet
C E P
SDS
1 ml

Sheep anti Human orexin A antibody recognizes the human orexin A subunit of orexin, also known as hypocretin. Orexin is cleaved into Orexin A, also known as hypocretin-1, a 32 amino acid excitatory neuropeptide linked to alertness and wakefulness in humans. Orexin A binds to both orexin receptors 1 and 2 with similar affinity whilst orexin B corresponding to the C terminal region of the holopeptide binds only to the orexin receptor 2.

Mutations in the orexin gene can lead to the development of Narcolepsy 1 (NRCLP1), a condition characterized by sleep fragmentation and daytime drowziness, abnormal REM sleep and sleep paralysis along with other sleep related symptoms (Peyron et al. 2000).

Sheep anti Human orexin A antibody is a useful reagent for immunohistochemistry on formalin fixed, paraffin embedded tissue sections.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey Expected from Sequence
Rat Expected from Sequence
Rabbit Expected from Sequence
Pig Expected from Sequence
Mouse Expected from Sequence
Horse Expected from Sequence
Dog Expected from Sequence
Chimpanzee Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human orexin A conjugated to KLH.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
O43612
Entrez Gene
HCRT
GO Terms
GO:0005576 extracellular region
GO:0005791 rough endoplasmic reticulum
GO:0007218 neuropeptide signaling pathway
GO:0008021 synaptic vesicle
GO:0030054 cell junction
Regulatory
For research purposes only

Applications of Orexin A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Hypothalamus

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

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