ORAI3 antibody

Rabbit anti ORAI3 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ORAI3
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1496
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SDS Safety Datasheet SDS
C WB 0.1 mg loader
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Rabbit anti Human ORAI3 antibody recognizes the N-terminal region of human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kDa multi-pass membrane protein belonging to the ORAI family.

Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.

ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2.

Rabbit anti Human ORAI3 antibody ( AHP1496) is not expected to cross react with either ORAI1 or ORAI2.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ORAI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 15 amino acid peptide from near the amino terminus of human ORAI3.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1496 detects a band of approximately 35kDa in A20 cell lysates.

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Further Reading

  1. Feske, S., et al. (2006) A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function.
    Nature; 441:179-85
  2. Soboloff, J. et al. (2006) Calcium signals mediated by STIM and Orai proteins--a new paradigm in inter-organelle communication.
    Biochim Biophys Acta. 1763 (11): 1161-8.
  3. Mercer, J.C. et al. (2006) Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1.
    J Biol Chem. 281 (34): 24979-90.
  4. Lewis, R.S. (2001) Calcium signaling mechanisms in T lymphocytes.
    Annu Rev Immunol. 19: 497-521.

RRID
AB_2157578
UniProt
Q9BRQ5
Entrez Gene
ORAI3
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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