Rabbit anti NFkB p65 (pSer536) antibody recognizes nuclear factor NF-kappa-B p65 subunit (NFkB p65), also known as nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 and transcription factor p65, when phosphorylated at serine 536. NFkB is a heterodimeric DNA binding protein which was originally identified consisting of p65 and p50 (NFkB1) subunits. Other subunits, including p52 (NFkB2), cRel, and RelB exist.
NFkB is ubiquitously expressed, and functions as a second messenger which upon activation leads to the transcription of a number of genes. NFkB is activated by a number of agents, including pro-inflammatory cytokines and bacterial lipopolysaccharide, and is essential for the maturation of T cells and regulation of their survival and activation.
Phosphorylation of serine 536 contributes to NFkB transactivation and increases p65 transcription activity.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to NFkB p65 (pSer536) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Synthetic peptide corresponding to residues surrounding phosphoserine 536 of human NFkB p65 protein.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific enhancer binding RNA polymerase II transcription factor activity
transcription factor complex
cellular defense response
Toll signaling pathway
identical protein binding
response to UV-B
transcription activator activity
transcription repressor activity
cytokine-mediated signaling pathway
protein kinase binding
membrane protein intracellular domain proteolysis
ubiquitin protein ligase binding
negative regulation of gene-specific transcription
activating transcription factor binding
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB cascade
interspecies interaction between organisms
innate immune response
positive regulation of transcription from RNA polymerase II promoter
protein N-terminus binding
nerve growth factor receptor signaling pathway
regulation of inflammatory response
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
defense response to virus
nucleotide-binding oligomerization domain containing 2 signaling pathway
repressing transcription factor binding
response to interleukin-1
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of NFkB p65 (pSer536) antibody
|Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human kidney distal tubules and collecting ducts.
- Western Blotting
- AHP1342 detects a band of approximately 65kDa in the appropriate cell lysate.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for NFkB p65 (pSer536) antibody
Doukas, P.G. et al. (2019) Temporal characteristics of NF-κB inhibition in blocking bile-induced oncogenic molecular events in hypopharyngeal cells.
Oncotarget. 10 (36): 3339-51.
Doukas, S.G. et al. (2020) The in vivo. preventive and therapeutic properties of curcumin in bile reflux-related oncogenesis of the hypopharynx.
J Cell Mol Med. 24 (18): 10311-21.
Mattioli, I. et al. (2004) Transient and selective NF-kappa B p65 serine 536 phosphorylation induced by T cell costimulation is mediated by I kappa B kinase beta and controls the kinetics of p65 nuclear import.
J Immunol. 172 (10): 6336-44.
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