NFkB p65 (pSer536) antibody

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Rabbit anti NFkB p65 (pSer536)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1342 E P WB* 50 µg
Rabbit anti NFkB p65 (pSer536) antibody recognizes nuclear factor NF-kappa-B p65 subunit (NFkB p65), also known as nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 and transcription factor p65, when phosphorylated at serine 536. NFkB is a heterodimeric DNA binding protein which was originally identified consisting of p65 and p50 (NFkB1) subunits. Other subunits, including p52 (NFkB2), cRel, and RelB exist.

NFkB is ubiquitously expressed, and functions as a second messenger which upon activation leads to the transcription of a number of genes. NFkB is activated by a number of agents, including pro-inflammatory cytokines and bacterial lipopolysaccharide, and is essential for the maturation of T cells and regulation of their survival and activation.

Phosphorylation of serine 536 contributes to NFkB transactivation and increases p65 transcription activity.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to NFkB p65 (pSer536) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to residues surrounding phosphoserine 536 of human NFkB p65 protein.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q04206 Related reagents
Entrez Gene
RELA Related reagents
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003682 chromatin binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006968 cellular defense response
GO:0008063 Toll signaling pathway
GO:0042802 identical protein binding
GO:0010224 response to UV-B
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0019221 cytokine-mediated signaling pathway
GO:0019901 protein kinase binding
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031625 ubiquitin protein ligase binding
GO:0032582 negative regulation of gene-specific transcription
GO:0033613 activating transcription factor binding
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042301 phosphate binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0047485 protein N-terminus binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050727 regulation of inflammatory response
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051607 defense response to virus
GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
GO:0070491 repressing transcription factor binding
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of NFkB p65 (pSer536) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/3000
Immunohistology - Paraffin 1/50 1/250
Western Blotting 1 1/100 1/1000
  1. 1 For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney distal tubules and collecting ducts.
Western Blotting
AHP1342 detects a band of approximately 65kDa in the appropriate cell lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

Further Reading

  1. Mattioli, I. et al. (2004) Transient and selective NF-kappa B p65 serine 536 phosphorylation induced by T cell costimulation is mediated by I kappa B kinase beta and controls the kinetics of p65 nuclear import.
    J Immunol. 172 (10): 6336-44.