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NFkB p65 (pSer536) antibody

Rabbit anti NFkB p65 (pSer536)

Product Type
Polyclonal Antibody
Polyclonal IgG
NFkB p65 (pSer536)

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P WB 50 µg loader
List Price Your Price

Rabbit anti NFkB p65 (pSer536) antibody recognizes nuclear factor NF-kappa-B p65 subunit (NFkB p65), also known as nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 and transcription factor p65, when phosphorylated at serine 536. NFkB is a heterodimeric DNA binding protein which was originally identified consisting of p65 and p50 (NFkB1) subunits. Other subunits, including p52 (NFkB2), cRel, and RelB exist.

NFkB is ubiquitously expressed, and functions as a second messenger which upon activation leads to the transcription of a number of genes. NFkB is activated by a number of agents, including pro-inflammatory cytokines and bacterial lipopolysaccharide, and is essential for the maturation of T cells and regulation of their survival and activation.

Phosphorylation of serine 536 contributes to NFkB transactivation and increases p65 transcription activity.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to NFkB p65 (pSer536) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Synthetic peptide corresponding to residues surrounding phosphoserine 536 of human NFkB p65 protein.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/3000
Immunohistology - Paraffin 1/50 1/250
Western Blotting 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney distal tubules and collecting ducts.
Western Blotting
AHP1342 detects a band of approximately 65kDa in the appropriate cell lysate.

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References for NFkB p65 (pSer536) antibody

  1. Doukas, P.G. et al. (2019) Temporal characteristics of NF-κB inhibition in blocking bile-induced oncogenic molecular events in hypopharyngeal cells.
    Oncotarget. 10 (36): 3339-51.
  2. Doukas, S.G. et al. (2020) The in vivo. preventive and therapeutic properties of curcumin in bile reflux-related oncogenesis of the hypopharynx.
    J Cell Mol Med. 24 (18): 10311-21.
  3. Vageli, D.P. et al. (2021) Targeting STAT3 prevents bile reflux-induced oncogenic molecular events linked to hypopharyngeal carcinogenesis.
    J Cell Mol Med. Dec 01 [Epub ahead of print].
  4. ElSayed, M.H. et al. (2023) Betanin improves motor function and alleviates experimental Parkinsonism via downregulation of TLR4/MyD88/NF-κB pathway: Molecular docking and biological investigations.
    Biomed Pharmacother. 164: 114917.
  5. Doukas, P.G. et al. (2021) Pepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells.
    Int J Mol Sci. 22 (8): 4275.

Further Reading

  1. Mattioli, I. et al. (2004) Transient and selective NF-kappa B p65 serine 536 phosphorylation induced by T cell costimulation is mediated by I kappa B kinase beta and controls the kinetics of p65 nuclear import.
    J Immunol. 172 (10): 6336-44.

Entrez Gene
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003682 chromatin binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006968 cellular defense response
GO:0008063 Toll signaling pathway
GO:0042802 identical protein binding
GO:0010224 response to UV-B
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0019221 cytokine-mediated signaling pathway
GO:0019901 protein kinase binding
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031625 ubiquitin protein ligase binding
GO:0032582 negative regulation of gene-specific transcription
GO:0033613 activating transcription factor binding
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042301 phosphate binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0047485 protein N-terminus binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050727 regulation of inflammatory response
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051607 defense response to virus
GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
GO:0070491 repressing transcription factor binding
GO:0070555 response to interleukin-1


148510 180604

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