NDUFS3 antibody

Rabbit anti NDUFS3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
NDUFS3

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VPA00285
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Rabbit anti Human NDUFS3 antibody recognizes NDUFS3 also known as CI-30kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, NADH dehydrogenase-ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit and complex I 30 kDa subunit.

The NDUFS3 gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in NDUFS3 gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency (provided by RefSeq, Apr 2009).

Rabbit anti Human NDUFS3 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti NDUFS3 detects a band of approximately 26 kDa in HEK293 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 251-284 of human NDUFS3
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
O75489
Entrez Gene
NDUFS3
GO Terms
GO:0009055 electron carrier activity
GO:0005515 protein binding
GO:0005747 mitochondrial respiratory chain complex I
GO:0006120 mitochondrial electron transport, NADH to ubiquinone
GO:0006800 oxygen and reactive oxygen species metabolic process
GO:0006810 transport
GO:0006917 induction of apoptosis
GO:0008137 NADH dehydrogenase (ubiquinone) activity
GO:0030308 negative regulation of cell growth
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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