Muscarinic Receptor M3 antibody
Mutations in the CHRM gene can lead to the development of Prune Belly Syndrome (PBS), a condition characterized by weak abdominal musculature, lax overlying skin, cryptorchism and urinary tract abnormalities (Weber et al. 2011).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human muscarinic receptor M3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to the 3rd cytoplasmic domain of human muscarinic receptor M3, conjugated to Keyhole Limpet Hemocyanin (KLH)
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20309
- Entrez Gene
- CHRM3
- GO Terms
- GO:0004435 phosphatidylinositol phospholipase C activity
- GO:0004981 muscarinic acetylcholine receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006112 energy reserve metabolic process
- GO:0006464 protein modification process
- GO:0007399 nervous system development
- GO:0050796 regulation of insulin secretion
- GO:0008283 cell proliferation
- GO:0016323 basolateral plasma membrane
- GO:0030054 cell junction
- GO:0045211 postsynaptic membrane
- Regulatory
- For research purposes only
Applications of Muscarinic Receptor M3 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin 1 | 10ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Brain, cortex.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
![]() |
|
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
Histar Detection System | STAR3000A | C P | 50 Tests |
Product Specific References
References for Muscarinic Receptor M3 antibody
-
Billington, C.K. & Penn, R.B. (2002) m3 muscarinic acetylcholine receptor regulation in the airway.
Am J Respir Cell Mol Biol. 26 (3): 269-72.
Further Reading
-
Felton, J. et al. (2018) Targeting M3 Muscarinic Receptors for Colon Cancer Therapy.
Curr Mol Pharmacol. Jan 18 [Epub ahead of print]. -
Pronin, A.N. et al. (2017) Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor.
Mol Pharmacol. 92 (5): 601-612.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra