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Muscarinic Receptor M3 antibody

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Staining of formalin fixed, paraffin embedded human brain cortex with Rabbit anti Human muscarinic receptor m3 (AHP1355)

Rabbit anti Human Muscarinic Receptor M3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Muscarinic Receptor M3

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Product Code Applications Pack Size List Price Your Price Qty
AHP1355
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * 50 µg loader
List Price Your Price
loader

Rabbit anti Human muscarinic receptor M3 antibody detects the muscarinic receptor M3, a G protein-coupled receptor for acetylcholine which is expressed in smooth muscle and glandular tissues. The M3 subtype is coupled to other G proteins known as Go or Gq which activate phospholipase C leading to an increase in intracellular Ca2+. The M3 muscarinic receptor controls smooth muscle contraction in blood vessels and lungs as well as in the gastrointestinal tract. It is also located in many glands, and its activation helps to stimulate secretion.

Mutations in the CHRM gene can lead to the development of Prune Belly Syndrome (PBS), a condition characterized by weak abdominal musculature, lax overlying skin, cryptorchism and urinary tract abnormalities (Weber et al. 2011).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human muscarinic receptor M3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the 3rd cytoplasmic domain of human muscarinic receptor M3, conjugated to Keyhole Limpet Hemocyanin (KLH)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain, cortex.

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Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
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Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
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Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
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Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0

References for Muscarinic Receptor M3 antibody

  1. Billington, C.K. & Penn, R.B. (2002) m3 muscarinic acetylcholine receptor regulation in the airway.
    Am J Respir Cell Mol Biol. 26 (3): 269-72.

Further Reading

  1. Felton, J. et al. (2018) Targeting M3 Muscarinic Receptors for Colon Cancer Therapy.
    Curr Mol Pharmacol. Jan 18 [Epub ahead of print].
  2. Pronin, A.N. et al. (2017) Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor.
    Mol Pharmacol. 92 (5): 601-612.

RRID
AB_906091
UniProt
P20309
Entrez Gene
CHRM3
GO Terms
GO:0004435 phosphatidylinositol phospholipase C activity
GO:0004981 muscarinic acetylcholine receptor activity
GO:0005887 integral to plasma membrane
GO:0006112 energy reserve metabolic process
GO:0006464 protein modification process
GO:0007399 nervous system development
GO:0050796 regulation of insulin secretion
GO:0008283 cell proliferation
GO:0016323 basolateral plasma membrane
GO:0030054 cell junction
GO:0045211 postsynaptic membrane

AHP1355

149684 155918 161170 180108

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