Muscarinic Receptor M3 antibody

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Rabbit anti Human Muscarinic Receptor M3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1355 P* 50 µg
Rabbit anti Human muscarinic receptor M3 antibody detects the muscarinic receptor M3, a G protein-coupled receptor for acetylcholine which is expressed in smooth muscle and glandular tissues. The M3 subtype is coupled to other G proteins known as Go or Gq which activate phospholipase C leading to an increase in intracellular Ca2+. The M3 muscarinic receptor controls smooth muscle contraction in blood vessels and lungs as well as in the gastrointestinal tract. It is also located in many glands, and its activation helps to stimulate secretion.

Mutations in the CHRM gene can lead to the development of Prune Belly Syndrome (PBS), a condition characterized by weak abdominal musculature, lax overlying skin, cryptorchism and urinary tract abnormalities (Weber et al. 2011).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human muscarinic receptor M3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the 3rd cytoplasmic domain of human muscarinic receptor M3, conjugated to Keyhole Limpet Hemocyanin (KLH)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P20309 Related reagents
Entrez Gene
CHRM3 Related reagents
GO Terms
GO:0004435 phosphatidylinositol phospholipase C activity
GO:0004981 muscarinic acetylcholine receptor activity
GO:0005887 integral to plasma membrane
GO:0006112 energy reserve metabolic process
GO:0006464 protein modification process
GO:0007399 nervous system development
GO:0050796 regulation of insulin secretion
GO:0008283 cell proliferation
GO:0016323 basolateral plasma membrane
GO:0030054 cell junction
GO:0045211 postsynaptic membrane
Regulatory
For research purposes only

Applications of Muscarinic Receptor M3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain, cortex.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

References for Muscarinic Receptor M3 antibody

  1. Billington, C.K. & Penn, R.B. (2002) m3 muscarinic acetylcholine receptor regulation in the airway.
    Am J Respir Cell Mol Biol. 26 (3): 269-72.

Further Reading

  1. Felton, J. et al. (2018) Targeting M3 Muscarinic Receptors for Colon Cancer Therapy.
    Curr Mol Pharmacol. Jan 18 [Epub ahead of print].
  2. Pronin, A.N. et al. (2017) Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor.
    Mol Pharmacol. 92 (5): 601-612.