MIP-3 Beta antibody

Rabbit anti Human MIP-3 Beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
MIP-3 Beta

Product Code Applications Pack Size List Price Your Price Qty
AHP789
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Human MIP-3 beta antibody recognizes human MIP-3 beta, (Macrophage Inflammatory Protein 3 beta), otherwise known as CCL19, a 77 amino acid CC chemokine expressed at high levels in the thymus and lymph nodes and to a lesser extent in the spleen, kidney, lungs, small intestine and stomach.

MIP-3 beta acts as a chemoattractant for T and B lymphocytes, dendritic cells, NK cells and myeloid progenitor cells, through specific binding to CCR7, a G-protein coupled receptor known to be up-regulated by Epstein-Barr virus infected B cells and herpesvirus infected T cells. A correlation between the response to MIP-3 beta and the level of CCR7 expression and has been revealed in the maturation of dendritic cells (DCs), and increased levels of both MIP-3 beta and CCR7 expression have been shown in bronchoalveolar lavage fluid (BALF) and BALF cells from patients with the granulomatous disorder sarcoidosis, associated predominantly with CD4+ T lymphocyte alveolitis, and hence T cell recruitment.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add azide.
Antiserum Preparation
Antisera to human MIP-3 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human MIP-3 beta.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 2.7 4.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Yoshida, R. et al. (1997) Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7.
    J Biol Chem. 272 (21): 13803-9.

Synonyms
CCL19
RRID
AB_808661
UniProt
Q99731
Entrez Gene
CCL19
GO Terms
GO:0009615 response to virus
GO:0007165 signal transduction
GO:0005615 extracellular space
GO:0006874 cellular calcium ion homeostasis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007154 cell communication
GO:0008009 chemokine activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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