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MIF antibody

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Anti MIF Antibody gallery image 1
Western blot analysis of Jurkat cell lysate (lane 1& 2), 3T3 cell lysate (lane 3) and rat kidney tissue lysate (lane 4) probed with Rabbit anti MIF antibody (MCA2328).

Rabbit anti MIF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2328
Datasheet
IP WB
SDS
50 µg loader

Rabbit anti MIF antibody recognizes macrophage migration inhibitory factor (MIF), also known referred to as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase. MIF is an important regulator of innate immunity. Bacterial antigens stimulate white blood cells to release MIF into the blood stream. The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. Hence, MIF is classified as an inflammatory cytokine

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
5mM EDTA
Immunogen
Synthetic peptide surrounding amino acid 67 of human MIF
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P14174
Entrez Gene
MIF
GO Terms
GO:0001516 prostaglandin biosynthetic process
GO:0004167 dopachrome isomerase activity
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0008283 cell proliferation
GO:0009986 cell surface
GO:0010629 negative regulation of gene expression
GO:0010739 positive regulation of protein kinase A signaling cascade
GO:0030890 positive regulation of B cell proliferation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0042056 chemoattractant activity
GO:0043030 regulation of macrophage activation
GO:0043066 negative regulation of apoptosis
GO:0043498 cell surface binding
GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045087 innate immune response
GO:0048146 positive regulation of fibroblast proliferation
GO:0050178 phenylpyruvate tautomerase activity
GO:0050715 positive regulation of cytokine secretion
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0070207 protein homotrimerization
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071157 negative regulation of cell cycle arrest
Regulatory
For research purposes only

Applications of MIF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 5 10 ug/ml
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti MIF antibody recognizes a band of approximately 12 kDa in Jurkat and rat kidney lysates
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
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Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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